Cellular Analysis and Comparative Transcriptomics Reveal the Tolerance Mechanisms of Candida tropicalis Toward Phenol

Frontiers in Microbiology

Xi Li¹  Likou Zou²  Yunfu Gu²  Quanju Xiang²  Xiumei Yu²  Xiaoping Zhang²  Qiang Chen²  Ke Zhao²  Xiaolin Kuang⁵  Ellen Ayepa⁵  Xuebing Han⁵  Menggen Ma⁵  Yuanyuan Peng⁵  Hanyu Wang⁵  Getachew Tafere Abrha⁵  Zhengyue Zhang⁵  Xiangdong Hu⁵  Qian Li⁵  Xiaoying Li⁶  Beidong Liu⁷ 

[1] College of Landscape Architecture, Sichuan Agricultural University, Chengdu, China;Department of Applied Microbiology, College of Resources, Sichuan Agricultural University, Chengdu, China;Department of Chemistry and Molecular Biology, University of Gothenburg, Göteburg, Sweden;F University, Hangzhou, China;Institute of Resources and Geographic Information Technology, College of Resources, Sichuan Agricultural University, Chengdu, China;School of Forestry and Life Science, Chongqing University of Arts and Sciences, Chongqing, China;;State Key Laboratory of Subtropical Silviculture, School of Forestry and Biotechnology, Zhejiang A&

关键词: Candida tropicalis;    morphological observation;    phenol;    reactive oxygen species (ROS);    tolerance mechanism;    transcriptome;   

DOI  :  10.3389/fmicb.2020.00544

来源: DOAJ

【 摘 要 】

Phenol is a ubiquitous pollutant and can contaminate natural water resources. Hence, the removal of phenol from wastewater is of significant importance. A series of biological methods were used to remove phenol based on the natural ability of microorganisms to degrade phenol, but the tolerance mechanism of phenol-degraded strains to phenol are not very clear. Morphological observation on Candida tropicalis showed that phenol caused the reactive oxygen species (ROS) accumulation, damaging the mitochondrial and the endoplasmic reticulum. On the basis of transcriptome data and cell wall susceptibility analysis, it was found that C. tropicalis prevented phenol-caused cell damage through improvement of cell wall resistance, maintenance of high-fidelity DNA replication, intracellular protein homeostasis, organelle integrity, and kept the intracellular phenol concentration at a low level through cell-wall remodeling and removal of excess phenol via MDR/MXR transporters. The knowledge obtained will promote the genetic modification of yeast strains in general to tolerate the high concentrations of phenol and improve their efficiency of phenol degradation.

【 授权许可】

Unknown