Improved hepatic γ-tocopherol status limits oxidative and inflammatory stress-mediated liver injury in db/db mice with nonalcoholic steatohepatitis

Journal of Functional Foods

Jinhui Li¹  J. David Symons²  Richard S. Bruno²  Eunice Mah³  Priyankar Dey³  Thunder Jalili³ 

[1] Biofortis, Inc., Addison, IL, USA;Department of Nutrition and Integrative Physiology, University of Utah, Salt Lake City, UT 84112, USA;Human Nutrition Program, The Ohio State University, Columbus, OH 43210, USA;

关键词: Diabetes;    Gamma-tocopherol;    iNOS;    Insulin resistance;    Lipid peroxidation;    Nonalcoholic steatohepatitis;   

DOI  :  

来源: DOAJ

【 摘 要 】

γ-Tocopherol (γ-T) and its physiological metabolite γ-carboxyethyl-hydroxychroman (γ-CEHC) exhibit antioxidant and anti-inflammatory activities. We hypothesized that improved hepatic γ-T status would protect against NASH in leptin-resistant db/db mice. Wild-type mice were fed a diet without γ-T, and db/db mice were fed the same diet supplemented with or without γ-T for 8 wk. Hepatic γ-T and γ-CEHC levels were increased (P < .05) compared with db/db controls. Serum ALT and insulin resistance, which were otherwise increased in db/db controls, were 33–38% lower in db/db mice fed γ-T. Increased hepatic lipid peroxidation in db/db mice was attenuated by dietary γ-T, which occurred without increasing hepatic Nrf2 mRNA expression. However, γ-T attenuated hepatic iNOS, MCP-1, TNF-α and CRP expression. Histological assessment showed γ-T-mediated improvements in overall hepatic architecture, specifically by limiting hepatic steatosis. These findings demonstrate that improvements in hepatic γ-T status protect against liver injury during NASH by reducing inflammation and oxidative stress.

【 授权许可】

Unknown