Experimental mixture design as a tool to optimize the growth of various Ganoderma species cultivated on media with different sugars

Mycology

Yit Kheng Goh¹  Kah Joo Goh¹  Suet Yee Tan¹  Nurul Fadhilah Marzuki¹  Swee Sian Tan¹  You Keng Goh¹  Hun Jiat Tung² 

[1] Advanced Agriecological Research Sdn Bhd;University of Nottingham Malaysia Campus (UNMC) Biotechnology Research Centre;

关键词: Cluster analysis;    coconut;    Malaysia;    mycelial growth;    simple sugars;    oil palm;   

DOI  :  10.1080/21501203.2015.1137985

来源: DOAJ

【 摘 要 】

The influence of different medium components (glucose, sucrose, and fructose) on the growth of different Ganoderma isolates and species was investigated using mixture design. Ten sugar combinations based on three simple sugars were generated with two different concentrations, namely 3.3% and 16.7%, which represented low and high sugar levels, respectively. The media were adjusted to either pH 5 or 8. Ganoderma isolates (two G. boninense from oil palm, one Ganoderma species from coconut palm, G. lingzhi, and G. australe from tower tree) grew faster at pH 8. Ganoderma lingzhi proliferated at the slowest rate compared to all other tested Ganoderma species in all the media studied. However, G. boninense isolates grew the fastest. Different Ganoderma species were found to have different sugar preferences. This study illustrated that the mixture design can be used to determine the optimal combinations of sugar or other nutrient/chemical components of media for fungal growth.

【 授权许可】

Unknown