Evaluation of a Novel Chromogenic Medium for the Detection of Pseudomonas aeruginosa in Respiratory Samples from Patients with Cystic Fibrosis

Microorganisms

Alexander Twist¹  Stephen P. Stanforth¹  Andrey Zaytsev¹  Thang V. Truong¹  Graeme Turnbull¹  John D. Perry¹  Audrey Perry²  Emma C. L. Marrs²  Sylvain Orenga³ 

[1] Department of Applied Sciences, Northumbria University, Newcastle upon Tyne NE1 8ST, UK;Microbiology Department, Freeman Hospital, Newcastle upon Tyne NE7 7DN, UK;Research & Development Microbiology, bioMérieux SA, 3 Route de Port Michaud, 38 390 La-Balme-les-Grottes, France;

关键词: Pseudomonas aeruginosa;    cystic fibrosis;    culture media;    chromogenic;    aminopeptidase;   

DOI  :  10.3390/microorganisms10051004

来源: DOAJ

【 摘 要 】

Pseudomonas aeruginosa is a dominant cause of respiratory infection in individuals with cystic fibrosis (CF), leading to significant morbidity and mortality. Detection of P. aeruginosa is conducted by culture of respiratory samples but this process may occasionally be compromised due to overgrowth by other bacteria and fungi. We aimed to evaluate a novel chromogenic medium, Pseudomonas aeruginosa chromogenic agar (PACA), for culture of P. aeruginosa from respiratory samples, from patients with CF. A total of 198 respiratory samples were cultured onto PACA and three other media: CHROMID^® P. aeruginosa, CHROMagar™ Pseudomonas and MacConkey agar. P. aeruginosa was recovered from 66 samples (33%), using a combination of all media. After 72 h incubation, the sensitivity of the four chromogenic media was as follows: 91% for PACA and CHROMagar™ Pseudomonas, 85% for CHROMID^® P. aeruginosa and 83% for MacConkey agar. For the three chromogenic media, the positive predictive value after 72 h was as follows: 95% for PACA, 56% for CHROMagar™ Pseudomonas and 86% for CHROMID^® P. aeruginosa. PACA proved to be a highly effective culture medium for the isolation and specific detection of P. aeruginosa from respiratory samples.

【 授权许可】

Unknown