ThPOK Inhibits Osteoclast Formation Via NFATc1 Transcription and Function

JBMR Plus

Steven Y Zou¹  Yongjia Li¹  Wei Zou¹  Nidhi Rohatgi¹  Steven L Teitelbaum¹  Takashi Izawa¹ 

[1] Division of Anatomic and Molecular Pathology, Department of Pathology and Immunology Washington University School of Medicine St. Louis MO USA;

关键词: OSTEOCLASTS;    CELLS OF BONE;    CELL/TISSUE SIGNALING;    TRANSCRIPTION FACTORS;    OSTEOPOROSIS;    DISEASES AND DISORDERS OF/RELATED TO BONE;   

DOI  :  10.1002/jbm4.10613

来源: DOAJ

【 摘 要 】

ABSTRACT Both LRF (Zbtb7a) and ThPOK (Zbtb7b) belong to the POK (BTB/POZ and Kruppel) family of transcription repressors that participate in development, differentiation, and oncogenesis. Although LRF mediates osteoclast differentiation by regulating NFATc1 expression, the principal established function of ThPOK is transcriptional control of T‐cell lineage commitment. Whether ThPOK affects osteoclast formation or function is not known. We find that marrow macrophage ThPOK expression diminishes with exposure to receptor activator of NF‐kB ligand (RANKL), but ThPOK deficiency does not affect osteoclast differentiation. On the other hand, enhanced ThPOK, in macrophages, substantially impairs osteoclastogenesis. Excess ThPOK binds the NFATc1 promoter and suppresses its transcription, suggesting a mechanism for its osteoclast inhibitory effect. Despite suppression of osteoclastogenesis by excess ThPOK being associated with diminished NFATc1, osteoclast formation is not rescued by NFATc1 overexpression. Thus, ThPOK appears to inhibit NFATc1 transcription and its osteoclastogenic capacity, while its depletion has no effect on the bone‐resorptive cell. © 2022 The Authors. JBMR Plus published by Wiley Periodicals LLC on behalf of American Society for Bone and Mineral Research.

【 授权许可】

Unknown