Expression and Functionality Study of 9 Toll-Like Receptors in 33 Drug-Naïve Non-Affective First Episode Psychosis Individuals: A 3-Month Study

International Journal of Molecular Sciences

Laura Riesco-Davila¹  Marcos Lopez-Hoyos²  JuanCarlos Leza³  Javier Vazquez-Bourgon⁴  Esther Setien-Suero⁴  Rosa Ayesa-Arriola⁴  Victor Ortiz-Garcia de la Foz⁴  Maria Juncal-Ruiz⁵  Jacqueline Mayoral-Van Son⁶  Benedicto Crespo-Facorro⁶ 

[1] Department of Immunology, Marques de Valdecilla University Hospital, IDIVAL, School of Medicine, University of Cantabria, 39008 Santander, Spain;Department of Immunology, Marques de Valdecilla University Hospital, PI of “Transplant and Autoimmunity” Research Group, REDINREN RD16/0027, IDIVAL, School of Medicine, University of Cantabria, 39008 Santander, Spain;Department of Pharmacology & Toxicology, Faculty of Medicine, Complutense University (UCM), Instituto de Investigación Sanitaria (IIS) Hospital 12 de Octubre (i+12), and IUIN-UCM, CIBERSAM, 28040 Madrid, Spain;Department of Psychiatry, Marques de Valdecilla University Hospital, IDIVAL, CIBERSAM, School of Medicine, University of Cantabria, 39008 Santander, Spain;Department of Psychiatry, Sierrallana Hospital, IDIVAL, CIBERSAM, School of Medicine, University of Cantabria, 39300 Torrelavega, Spain;Department of Psychiatry, Virgen del Rocio University Hospital, Instituto de Investigacion Sanitaria de Sevilla (IBiS), CIBERSAM, School of Medicine, University of Sevilla, 41013 Sevilla, Spain;

关键词: psychosis;    neuroinflammation;    toll-like receptors;    cytokines;   

DOI  :  10.3390/ijms21176106

来源: DOAJ

【 摘 要 】

Toll-like receptors (TLRs) are a pivotal component of the innate immune system that seem to have a role in the pathogenesis of psychosis. The purpose of this work was to compare the expression and functionality of 9 TLRs in three peripheral blood mononuclear cells (PBMCs) (monocytes, B cells, and T cells) between 33 drug-naïve first-episode psychosis (FEP) individuals and 26 healthy volunteers, at baseline and after 3-month of antipsychotic treatment. The expression of TLRs 1–9 were assessed by flow cytometry. For the assessment of the TLR functionality, cells collected in sodium heparin tubes were polyclonally stimulated for 18 h, with different agonists for human TLR1–9. The results of our study highlight the role that TLR5 and TLR8 might play in the pathophysiology of psychosis. We found a lower expression of these receptors in FEP individuals, regarding healthy volunteers at baseline and after 3-month of treatment on the three PBMCs subsets. Most TLRs showed a lower functionality (especially reduced intracellular levels of TNF-α) in patients than in healthy volunteers. These results, together with previous evidence, suggest that individuals with psychosis might show a pattern of TLR expression that differs from that of healthy volunteers, which could vary according to the intensity of immune/inflammatory response.

【 授权许可】

Unknown