The formation of the light-sensing compartment of cone photoreceptors coincides with a transcriptional switch

eLife

Özkan Keles¹  Botond Roska¹  Sjoerd JB Holwerda²  Filippo M Rijli²  Hubertus Kohler²  Michael Stadler²  Janine M Daum² 

[1] University of Basel, Basel, Switzerland;Friedrich Miescher Institute for Biomedical Research, Basel, Switzerland;

关键词: retinal development;    cones;    RNA seq;   

DOI  :  10.7554/eLife.31437

来源: DOAJ

【 摘 要 】

High-resolution daylight vision is mediated by cone photoreceptors. The molecular program responsible for the formation of their light sensor, the outer segment, is not well understood. We correlated daily changes in ultrastructure and gene expression in postmitotic mouse cones, between birth and eye opening, using serial block-face electron microscopy (EM) and RNA sequencing. Outer segments appeared rapidly at postnatal day six and their appearance coincided with a switch in gene expression. The switch affected over 14% of all expressed genes. Genes that switched off were rich in transcription factors and neurogenic genes. Those that switched on contained genes relevant for cone function. Chromatin rearrangements in enhancer regions occurred before the switch was completed, but not after. We provide a resource comprised of correlated EM, RNAseq, and ATACseq data, showing that the growth of a key compartment of a postmitotic cell involves an extensive switch in gene expression and chromatin accessibility.

【 授权许可】

Unknown