The novel role of β-aescin in attenuating CCl4-induced hepatotoxicity in rats

Pharmaceutical Biology

Harsimran Singh¹  Shabir Sidhu²  M. U. Khan³  Kanwaljit Chopra⁴ 

[1] IKG Punjab Technical University;Punjab Institute of technology, IKG Punjab Technical University;Sri Sai College of Pharmacy, Badhani;University Institute of Pharmaceutical Sciences, UGC Centre of Advanced Study, Punjab University;

关键词: carbon tetrachloride;    silymarin;    antifibrotic;    oxidative stress;    nitrosative stress;    aesculus hippocastanum;   

DOI  :  10.1080/13880209.2016.1275023

来源: DOAJ

【 摘 要 】

Context: β-Aescin has anti-inflammatory, anti-oxidant and antiedematous properties. Objective: The present study investigated the hepatoprotective effect and underlying mechanisms of β-aescin in CCl4-induced liver damage. Materials and methods: Thirty-five Wistar rats were divided into six groups: normal control, CCl4 control, silymarin (50 mg/kg, p.o) and β-aescin (0.9, 1.8 and 3.6 mg/kg, i.p.) treatment for 14 d. CCl4 (1 mL/kg, i.p. for 3 d) was administered to produce hepatic damage. Ponderal changes and liver marker enzymes were estimated. Hepatic oxidative and nitrosative stress was estimated by levels of thiobarbituric acid reactive substances (TBARS), glutathione (GSH) and nitrite/nitrate. Serum TGF-β1 and TNF-α were estimated by ELISA technique. Hepatic collagen and histopathological studies were carried out. Results: β-Aescin (3.6 mg/kg) markedly decreased CCl4-induced increased levels of ALT, AST, ALP (71.77 versus 206.7, 71.39 versus 171.82, 121.20 versus 259 IU/L, respectively), total bilirubin (0.41 versus 1.35 mg/dL), TBARS (2.0 versus 8.83 nmol MDA/mg protein), nitrite/nitrate (352.50 versus 745.15 μg/mL) and increased CCl4-induced decreased GSH levels (0.095 versus 0.048 μmol/mg protein). β-Aescin (3.6 mg/kg) induced focal regenerative changes in liver and markedly decreased TBARS (2.0 versus 8.83 nmol MDA/mg protein), nitrite/nitrate (352.50 versus 745.15 μg/mL), TGF-β1 (92.28 versus 152.1 pg/mL), collagen content (110.75 versus 301.74 μmol/100 mg tissue) and TNF-α (92.82 versus 170.56 pg/mL) when compared with CCl4 control. Discussion and conclusion: The findings suggest that β-aescin has a protective effect on CCl4-induced liver injury, exhibited via its anti-inflammatory, antioxidative, antinitrosative and antifibrotic properties inducing repair regeneration of liver. Hence, it can be used as a promising hepatoprotective agent.

【 授权许可】

Unknown