Mitochondria do not contain lipid rafts, and lipid rafts do not contain mitochondrial proteins[S]

Journal of Lipid Research

Kyra B. Berg¹  Leonard J. Foster¹  Yu Zi Zheng¹ 

[1] Centre for High-Throughput Biology and Department of Biochemistry and Molecular Biology, University of British Columbia, Vancouver, BC, Canada, V6T 1Z4;

关键词: detergent-resistant membranes;    membrane proteins;    stable isotope labeling by amino acids in cell culture;    quantitative proteomics;    mass spectrometry;    methyl-β-cyclodextrin;   

DOI  :  

来源: DOAJ

【 摘 要 】

Lipid rafts are membrane microdomains involved in many cellular functions, including transduction of cellular signals and cell entry by pathogens. Lipid rafts can be enriched biochemically by extraction in a nonionic detergent at low temperature, followed by floatation on a sucrose density gradient. Previous proteomic studies of such detergent-resistant membranes (DRMs) are in disagreement about the presence of mitochondrial proteins in raft components. Here, we approach the status of mitochondrial proteins in DRM preparations by employing stable isotope labeling by amino acids in cell culture to evaluate the composition of differentially purified subcellular fractions as well as high-resolution linear density gradients. Our data demonstrate that F1/F0 ATPase subunits, voltage-dependent anion selective channels, and other mitochondrial proteins are at best partially copurifying contaminants of raft preparations.

【 授权许可】

Unknown