Partial silencing of fucosyltransferase 8 gene expression inhibits proliferation of Ishikawa cells, a cell line of endometrial cancer

Biochemistry and Biophysics Reports

Masahiko Ito¹  Hiroaki Itoh²  Hana Shimoyama³  Masashi Adachi³  Tetsuro Suzuki³  Hirotake Murakami³  Takeshi Nakayama³  Madoka Matsuya-Ogawa³  Toshiya Itoh³  Toshiaki K. Shibata³  Mari Mukai³  Kazuhiro Sugihara³  Naohiro Kanayama⁴  Tomoaki Oda⁴ 

[1] Corresponding author. Hamamatsu University School of Medicine, Department of Obstetrics and Gynecology, 1-20-1 Handayama, Higashi-ku, Hamamatsu, 431-3192, Japan.;Department of Faculty of Medical Technology, Fujita Health University, 1-98 Dengakugakubo, Kutsukake-cho, Toyoake, Aichi, 470-1192, Japan;Department of Obstetrics and Gynecology, Hamamatsu University School of Medicine, 1-20-1 Handayama, Higashi-ku, Hamamatsu, Shizuoka, 431-3192, Japan;Department of Virology and Parasitology, Hamamatsu University School of Medicine, 1-20-1 Handayama, Higashi-ku, Hamamatsu, Shizuoka, 431-3192, Japan;

关键词: Endometrial endometrioid carcinoma;    Fucosyltransferase 8;    Ishikawa cells;    Cell proliferation;    xCELLigence;   

DOI  :  

来源: DOAJ

【 摘 要 】

Endometrial cancer is the most common gynecologic malignancy and is associated with increased morbidity each year, including young people. However, its mechanisms of proliferation and progression are not fully elucidated. It is well known that abnormal glycosylation is involved in oncogenesis, and fucosylation is one of the most important types of glycosylation. In particular, fucosyltransferase 8 (FUT8) is the only FUT responsible for α1, 6-linked fucosylation (core fucosylation), and it is involved in various physiological as well as pathophysiological processes, including cancer biology. Therefore, we aimed to identify the expression of FUT8 in endometrial endometrioid carcinoma and investigate the effect of the partial silencing of the FUT8 gene on the cell proliferation of Ishikawa cells, an epithelial-like endometrial cancer cell line. Quantitative real-time PCR analysis showed that FUT8 gene expression was significantly elevated in the endometrial endometrioid carcinoma, compared to the normal endometrium. The immunostaining of FUT8 and Ulex europaeus Agglutinin 1 (UEA-1), a kind of lectin family specifically binding to fucose, was detected endometrial endometrioid carcinoma. The proliferation assay showed FUT8 partial knockdown by transfection of siRNA significantly suppressed the proliferation of Ishikawa cells, concomitant with the upregulation in the gene expressions associated with the interesting pathways associated with de-ubiquitination, aspirin trigger, mesenchymal-epithelial transition (MET) et al. It was suggested that the core fucosylation brought about by FUT8 might be involved in the proliferation of endometrial endometrioid carcinoma cells.

【 授权许可】

Unknown