期刊论文详细信息
Foods
Role of Lactic Acid Bacteria Phospho-β-Glucosidases during the Fermentation of Cereal by-Products
Pasquale Filannino1  Kashika Arora2  Marco Gobbetti2  AlessioDa Ros2  Raffaella Di Cagno2  Marta Acin-Albiac2 
[1] Department of Soil, Plant and Food Science, University of Bari Aldo Moro, 70126 Bari, Italy;Faculty of Science and Technology, Libera Universitá di Bolzano, 39100 Bolzano, Italy;
关键词: phospho-beta-glucosidases;    cereal by-products;    brewers’ spent grain;    lactic acid bacteria metabolism;   
DOI  :  10.3390/foods10010097
来源: DOAJ
【 摘 要 】

Bioprocessing using lactic acid bacteria (LAB) is a powerful means to exploit plant-derived by-products as a food ingredient. LAB have the capability to metabolize a large variety of carbohydrates, but such metabolism only relies on few metabolic routes, conferring on them a high fermentation potential. One example of these pathways is that involving phospho-β-glucosidase genes, which are present in high redundancy within LAB genomes. This enzymatic activity undertakes an ambivalent role during fermentation of plant-based foods related to the release of a wide range of phenolic compounds, from their β-D-glycosylated precursors and the degradation of β-glucopyranosyl derived carbohydrates. We proposed a novel phenomic approach to characterize the metabolism drift of Lactiplantibacillus plantarum and Leuconostoc pseudomesenteroides caused by a lignocellulosic by-product, such as the brewers’ spent grain (BSG), in contrast to Rich De Man, Rogosa and Sharpe (MRS) broth. We observed an increased metabolic activity for gentiobiose, cellobiose and β-glucoside conjugates of phenolic compounds during BSG fermentation. Gene expression analysis confirmed the importance of cellobiose metabolism while a release of lignin-derived aglycones was found during BSG fermentation. We provided a comprehensive view of the important role exerted by LAB 6-phospho-β-glucosidases as well the major metabolic routes undertaken during plant-based fermentations. Further challenges will consider a controlled characterization of pbg gene expression correlated to the metabolism of β-glucosides with different aglycone moieties.

【 授权许可】

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