5′,8-Cyclopurine Lesions in DNA Damage: Chemical, Analytical, Biological, and Diagnostic Significance

Cells

PawlosS. Tsegay¹  Yuan Liu²  NicholasE. Geacintov³  Vladimir Shafirovich³  MariosG. Krokidis⁴  Chryssostomos Chatgilialoglu⁵  Annalisa Masi⁵  Carla Ferreri⁵  MichaelA. Terzidis⁶ 

[1] Biochemistry Ph.D. Program, Florida International University, Miami, FL 33199, USA;Department of Chemistry and Biochemistry, Florida International University, 11200 SW 8th Street, Miami, FL 33199, USA;Department of Chemistry, New York University, 31 Washington Place, New York, NY 10003-5180, USA;Institute of Nanoscience and Nanotechnology, N.C.S.R. “Demokritos”, 15310 Agia Paraskevi Attikis, Greece;Istituto per la Sintesi Organica e la Fotoreattività, Consiglio Nazionale delle Ricerche, Via P. Gobetti 101, 40129 Bologna, Italy;Laboratory of Chemical and Environmental Technology, Department of Chemistry, Aristotle University of Thessaloniki, 54124 Thessaloniki, Greece;

关键词: reactive oxygen species;    free radicals;    DNA damage;    cyclopurines;    DNA and RNA polymerases;    nucleotide excision repair;    LC-MS/MS;    xeroderma pigmentosum;    cancer;   

DOI  :  10.3390/cells8060513

来源: DOAJ

【 摘 要 】

Purine 5′,8-cyclo-2′-deoxynucleosides (cPu) are tandem-type lesions observed among the DNA purine modifications and identified in mammalian cellular DNA in vivo. These lesions can be present in two diasteroisomeric forms, 5′R and 5′S, for each 2′-deoxyadenosine and 2′-deoxyguanosine moiety. They are generated exclusively by hydroxyl radical attack to 2′-deoxyribose units generating C5′ radicals, followed by cyclization with the C8 position of the purine base. This review describes the main recent achievements in the preparation of the cPu molecular library for analytical and DNA synthesis applications for the studies of the enzymatic recognition and repair mechanisms, their impact on transcription and genetic instability, quantitative determination of the levels of lesions in various types of cells and animal model systems, and relationships between the levels of lesions and human health, disease, and aging, as well as the defining of the detection limits and quantification protocols.

【 授权许可】

Unknown