SaHsfA4c From Sedum alfredii Hance Enhances Cadmium Tolerance by Regulating ROS-Scavenger Activities and Heat Shock Proteins Expression

Frontiers in Plant Science

Shuangshuang Chen¹  Renying Zhuo²  Yunxing Zhang²  Mingying Liu²  Miao Yu²  He Li²  Xiaojiao Han²  Ying Wang²  Guirong Qiao²  Zhuchou Lu²  Longhua Wu³ 

[1] Institute of Leisure Agriculture, Jiangsu Academy of Agricultural Sciences, Nanjing, China;Key Laboratory of Tree Breeding of Zhejiang Province, The Research Institute of Subtropical Forestry, Chinese Academy of Forestry, Hangzhou, China;National Engineering Laboratory of Soil Pollution Control and Remediation Technologies, Institute of Soil Science, Chinese Academy of Sciences, Nanjing, China;State Key Laboratory of Tree Genetics and Breeding, Chinese Academy of Forestry, Beijing, China;

关键词: Sedum alfredii Hance;    heat shock transcription factor;    cadmium stress;    overexpression;    ROS-scavenging enzyme;   

DOI  :  10.3389/fpls.2020.00142

来源: DOAJ

【 摘 要 】

The heat shock transcription factor (Hsf) family, an important member in plant stress response, affects cadmium (Cd) tolerance in plants. In this study, we identified and functionally characterized a transcript of the Hsf A4 subgroup from Sedum alfredii. Designated as SaHsfA4c, the open reading frame was 1,302 bp long and encoded a putative protein of 433 amino acids containing a complete DNA-binding domain (DBD). Heterologous expression of SaHsfA4c in yeast enhanced Cd stress tolerance and accumulation, whereas expression of the alternatively spliced transcript InSaHsfA4c which contained an intron and harbored an incomplete DBD, resulted in relatively poor Cd stress tolerance and low Cd accumulation in transgenic yeast. The function of SaHsfA4c under Cd stress was characterized in transgenic Arabidopsis and non-hyperaccumulation ecotype S. alfredii. SaHsfA4c was able to rescue the Cd sensitivity of the Arabidopsis athsfa4c mutant. SaHsfA4c reduced reactive oxygen species (ROS) accumulation and increased the expression of ROS-scavenging enzyme genes and Hsps in transgenic lines. The present results suggest that SaHsfA4c increases plant resistance to stress by up-regulating the activities of ROS-scavenging enzyme and the expression of Hsps.

【 授权许可】

Unknown