CENP-A Ubiquitylation Contributes to Maintaining the Chromosomal Location of the Centromere

Molecules

Yohei Niikura,¹  RisaKitagawa¹  Katsumi Kitagawa¹ 

[1] Greehey Children’s Cancer Research Institute, Department of Molecular Medicine, UT Health Science Center San Antonio School of Medicine, 8403 Floyd Curl Drive, San Antonio, TX 78229-3000, USA;

关键词: CENP-A;    centromere identity;    centromere;    CUL4A-RBX1- COPS8 E3 ligase;    dimerization;    epigenetics;    kinetochore;    lysine 124 (K124), neocentromere;    ubiquitylation;   

DOI  :  10.3390/molecules24030402

来源: DOAJ

【 摘 要 】

The centromere plays an essential role in accurate chromosome segregation, and the chromosomal location of the centromere is determined by the presence of a histone H3 variant, centromere protein A (CENP-A), in centromeric nucleosomes. However, the precise mechanisms of deposition, maintenance, and inheritance of CENP-A at centromeres are unclear. We have reported that CENP-A deposition requires ubiquitylation of CENP-A lysine 124 mediated by the E3 ligase activity of Cullin 4A (CUL4A)—RING-box protein 1 (RBX1)—COP9 signalsome complex subunit 8 (COPS8). We have proposed a model of inheritance for CENP-A ubiquitylation, through dimerization between rounds of cell divisions, that maintains the position of centromeres.

【 授权许可】

Unknown