Frontiers in Physiology | |
A Rhodopsin-Like Gene May Be Associated With the Light-Sensitivity of Adult Pacific Oyster Crassostrea gigas | |
Wen Guo1  Zhongqiang Cai2  Changlu Wu3  Lei Wei3  Jiao Wang3  Jun Chen3  Xiaotong Wang3  Wenchao Yu3  Qiuyun Jiang3  Cheng He3  | |
[1] Center for Mollusc Study and Development, Marine Biology Institute of Shandong Province, Qingdao, China;Changdao Enhancement and Experiment Station, Chinese Academy of Fishery Sciences, Changdao, China;School of Agriculture, Ludong University, Yantai, China; | |
关键词: adult oyster; light-sensitivity; RNAi; mantle; rhodopsin-like superfamily member gene; | |
DOI : 10.3389/fphys.2018.00221 | |
来源: DOAJ |
【 摘 要 】
Light-sensitivity is important for mollusc survival, as it plays a vital role in reproduction and predator avoidance. Light-sensitivity has been demonstrated in the adult Pacific oyster Crassostrea gigas, but the genes associated with light-sensitivity remain unclear. In the present study, we designed experiments to identify the genes associated with light-sensitivity in adult oysters. First, we assessed the Pacific oyster genome and identified 368 genes annotated with the terms associated with light-sensitivity. Second, the function of the four rhodopsin-like superfamily member genes was tested by using RNAi. The results showed that the highest level of mRNA expression of the vision-related genes was in the mantle; however, this finding is not true for all oyster genes. Interestingly, we also found four rhodopsin-like superfamily member genes expressed at an very high level in the mantle tissue. In the RNAi experiment, when one of rhodopsin-like superfamily member genes (CGI_1001253) was inhibited, the light-sensitivity capacity of the injected oysters was significantly reduced, suggesting that CGI_10012534 may be associated with light-sensitivity in the adult Pacific oyster.
【 授权许可】
Unknown