Transfection of Axin Gene Down-regulates Expressions of β-catenin and TCF-4 and Inhibits the Proliferation and Invasive Ability of Lung Cancer Cells

Chinese Journal of Lung Cancer

关键词: Axin protein;    Beta Catenin;    T cell factor 4;    Lung neoplasms;    Cell proliferation;   

DOI  :  

来源: DOAJ

【 摘 要 】

Background and objective Axin is an important negative regulator of Wnt signaling pathway. It can induce the phosphorylation and degradation of β-catenin. The reduced expression of axin or high expression of β-catenin and TCF-4 were associated with malignant proliferation in many tumors. The aim of this study is to examinethe relationships among the expressions and locations of axin, β-catenin and other relevant molecules, and the roles of axin on proliferation, invasive ability and apoptosis of lung cancer cells. Methods The axin cDNA was transfected into lung cancer BE1 cell line which has very low axin expression. The levels of expression and location of axin, β-catenin and TCF-4 before and after transfection were detected using immunofluorescence. The mRNA levels of expression of axin, β-catenin and TCF-4 were examined using reverse transcription-polymerase chain reaction (RT-PCR). The apoptosis,proliferation and invasive ability of lung cancer cells before and after transfection were examined with flow cytometry, MTTand transwell methods. Results After transfection of axin gene into BE1 cells (BE1-axin cells), axin mRNA and protein were overexpressed significantly. Meanwhile, the protein expression of β-catenin and mRNA expression of TCF-4 were decreased significantly in BE1-axin cells than that in BE1 or vector control cells. The flow cytometry revealed that the apoptosis rate of BE1-axin cells was enhanced, but MTT and Transwell assay indicated that the proliferation andinvasive ability were decreased significantly in BE1-axin cells than those in BE1 or vector control cells. Conclusion The overexpression of axin could down-regulate the protein expression of β-catenin and the transcription of TCF-4, and inhibit the proliferation and invasive ability of lung cancer cells.

【 授权许可】

Unknown