Genome-Wide Identification and Expression of MAPK Gene Family in Cultivated Strawberry and Their Involvement in Fruit Developing and Ripening

International Journal of Molecular Sciences

Min Yang¹  Ya Luo¹  Qing Chen¹  Wen He¹  Yong Zhang¹  Guoyan Hou¹  Yuanxiu Lin¹  Binghua Li¹  Xiaorong Wang¹  Yunting Zhang¹  Mengyao Li¹  Yan Wang¹  Liangxin Wang¹  Haoru Tang¹  Guichuan Yang² 

[1] College of Horticulture, Sichuan Agricultural University, Chengdu 611130, China;Departmental and Municipal Co-Construction of Crops Genetic Improvement of Hill Land Key Laboratory of Sichuan, Nanchong 637000, China;

关键词: cultivated strawberry;    mitogen-activated protein kinases;    genome-wide analysis;    expression pattern;    evolution;    ABA and sucrose treatment;   

DOI  :  10.3390/ijms23095201

来源: DOAJ

【 摘 要 】

Studies on many plants have shown that mitogen-activated protein kinases (MAPKs) are key proteins involved in regulating plant responses to biotic and abiotic stresses. However, their involvement in cultivated strawberry development and ripening remains unclear. In this study, 43 FaMAPK gene family members were identified in the genome of cultivated strawberry (Fragaria × ananassa), phylogenetic analysis indicated that FaMAPKs could be classified into four groups. Systematic analysis of the conserved motif, exon–intron structure showed that there were significant varieties between different groups in structure, but in the same group they were similar. Multiple cis-regulatory elements associated with phytohormone response, and abiotic and biotic stresses were predicted in the promoter regions of FaMAPK genes. Transcriptional analysis showed that all FaMAPK genes were expressed at all developmental stages. Meanwhile, the effect of exogenous ABA and sucrose on the expression profile of FaMAPKs was investigated. Exogenous ABA, sucrose, and ABA plus sucrose treatments upregulated the expression of FaMAPK genes and increased the content of endogenous ABA, sucrose, and anthocyanin in strawberry fruits, suggesting that ABA and sucrose might be involved in the FaMAPK-mediated regulation of strawberry fruit ripening. Based on the obtained results, MAPK genes closely related to the ripening of strawberries were screened to provide a theoretical basis and support for future research on strawberries.

【 授权许可】

Unknown