Enhanced Tolerance against a Fungal Pathogen and Insect Resistance in Transgenic Tobacco Plants Overexpressing an Endochitinase Gene from Serratia marcescens

International Journal of Molecular Sciences

Guadalupe Peña-Chora¹  LauraPatricia Lina-García²  VíctorManuel Hernández-Velázquez²  JoséAugusto Ramírez-Trujillo²  SamanthaSarai Navarro-González²  Ramón Suárez-Rodríguez²  Abigail Roldán-Salgado³  Gerardo Corzo³  Paul Gaytán³ 

[1] Centro de Investigaciones Biológicas, Universidad Autónoma del Estado de Morelos, Av. Universidad 1001, Col. Chamilpa, Cuernavaca, Morelos 62209, Mexico;Centro de Investigación en Biotecnología, Universidad Autónoma del Estado de Morelos, Av. Universidad 1001, Col. Chamilpa, Cuernavaca, Morelos 62209, Mexico;Instituto de Biotecnología, Universidad Nacional Autónoma de México, Av. Universidad 2001, Col. Chamilpa, Cuernavaca, Morelos 62210, Mexico;

关键词: Serratia marcescens;    bacterial chitinase;    antifungal activity;    transgenic plants;    Spodoptera frugiperda;   

DOI  :  10.3390/ijms20143482

来源: DOAJ

【 摘 要 】

In this study we cloned a chitinase gene (SmchiC), from Serratia marcescens isolated from the corpse of a Diatraea magnifactella lepidopteran, which is an important sugarcane pest. The chitinase gene SmchiC amplified from the S. marcescens genome was cloned into the transformation vector p2X35SChiC and used to transform tobacco (Nicotiana tabacum L. cv Petit Havana SR1). The resistance of these transgenic plants to the necrotrophic fungus Botrytis cinerea and to the pest Spodoptera frugiperda was evaluated: both the activity of chitinase as well as the resistance against B. cinerea and S. frugiperda was significantly higher in transgenic plants compared to the wild-type.

【 授权许可】

Unknown