Some properties of active and latent catechol oxidase of mushroom

Acta Agrobotanica

Janusz Czapski¹ 

[1] Research Institute of Vegetable Crops in Skierniewice;

关键词: Agaricus bisporus;    mushroom;    latent and active catechol oxidase;    isoenzyme pattern;   

DOI  :  10.5586/aa.1999.014

来源: DOAJ

【 摘 要 】

Latent form of mushroom catechol oxidase was activated by O,1% sodium dodecyl sulfate (SDS). Catalytic power of the latent form, calculated from the kinetic parameters was 1,8 times higher than that of active one. Salicyl hydroxamic acid (SHAM) appeared as a powerful inhibitor for both active and latent forms ofcatechol oxidase. However, in the range of 150-250 μM SHAM the inhibitory effect for active catechol oxidase was significantly higher than that for the latent one. Non-competitive and irreversible characteristics of inhibition of latent and active catechol oxidase was calculated from kinetic data. Electrophoretic analysis followed by scanning of the gels was used. The spots' absorbance was determined from a computer image of the isoenzyme band patterns. It allowed us to estimate gels quantitatively. Presence of one additional clearly defined slow moving isoform of SDS-activated catechol oxidase, differed in the respect of 3 bands for the active and 4 bands for the total.

【 授权许可】

Unknown