Sensitive Detection of Single-Nucleotide Polymorphisms by Solid Nanopores Integrated With DNA Probed Nanoparticles

Frontiers in Bioengineering and Biotechnology

Ling Zhi Wu¹  Zhi Xuan Wang¹  Li Xing Weng¹  Li Li²  Yuan Ye²  Bi Qing Bao²  Die Ma²  Guo Hao Xi² 

[1] College of Geography and Biological Information, Nanjing University of Posts and Telecommunications, Nanjing, China;Key Laboratory for Organic Electronics and Information Displays, Jiangsu Key Laboratory for Biosensors, Institute of Advanced Materials, National Synergetic Innovation Center for Advanced Materials, Nanjing University of Posts and Telecommunications, Nanjing, China;

关键词: single nucleotide polymorphism;    gold nanoparticle;    gastric cancer;    solid nanopore;    DNA probe;   

DOI  :  10.3389/fbioe.2021.690747

来源: DOAJ

【 摘 要 】

Single-nucleotide polymorphisms (SNPs) are the abundant forms of genetic variations, which are closely associated with serious genetic and inherited diseases, even cancers. Here, a novel SNP detection assay has been developed for single-nucleotide discrimination by nanopore sensing platform with DNA probed Au nanoparticles as transport carriers. The SNP of p53 gene mutation in gastric cancer has been successfully detected in the femtomolar concentration by nanopore sensing. The robust biosensing strategy offers a way for solid nanopore sensors integrated with varied nanoparticles to achieve single-nucleotide distinction with high sensitivity and spatial resolution, which promises tremendous potential applications of nanopore sensing for early diagnosis and disease prevention in the near future.

【 授权许可】

Unknown