Model Systems for Evidencing the Mediator Role of Riboflavin in the UVA Cross-Linking Treatment of Keratoconus

Molecules

Raluca Şomoghi¹  Bogdan Trică²  Marin Micutz³  Teodora Staicu³  Cătălina Gabriela Corbu⁴  Mihaela Monica Constantin⁴  Elena Irina Popescu⁵  Aurica Precupas⁵  Vlad Tudor Popa⁵  Gabriela Ionita⁵  Sorin Mocanu⁵  Iulia Matei⁵ 

[1] Chemistry Department, Faculty of Petroleum Technology and Petrochemistry, Petroleum-Gas University of Ploiesti, Bd. Bucuresti 39, 100680 Ploiesti, Romania;Department of Bioresources, National Institute for Research & Development in Chemistry and Petrochemistry—ICECHIM, Splaiul Independentei nr. 202, Sector 6, 060021 Bucharest, Romania;Department of Physical Chemistry, Faculty of Chemistry, University of Bucharest, Bd. Regina Elisabeta 4-12, 030018 Bucharest, Romania;Oftaclinic Clinic, Bd. Marasesti 2B, 040254 Bucharest, Romania;“Ilie Murgulescu” Institute of Physical Chemistry of the Romanian Academy, Splaiul Independentei 202, 060021 Bucharest, Romania;

关键词: collagen;    riboflavin;    hyaluronic acid;    EPR spectroscopy;    keratoconus;    STEM;   

DOI  :  10.3390/molecules27010190

来源: DOAJ

【 摘 要 】

Riboflavin under UVA radiation generates reactive oxygen species (ROS) that can induce various changes in biological systems. Under controlled conditions, these processes can be used in some treatments for ocular or dermal diseases. For instance, corneal cross-linking (CXL) treatment of keratoconus involves UVA irradiation combined with riboflavin aiming to induce the formation of new collagen fibrils in cornea. To reduce the damaging effect of ROS formed in the presence of riboflavin and UVA, the CXL treatment is performed with the addition of polysaccharides (dextran). Hyaluronic acid is a polysaccharide that can be found in the aqueous layer of the tear film. In many cases, keratoconus patients also present dry eye syndrome that can be reduced by the application of topical solutions containing hyaluronic acid. This study presents physico-chemical evidence on the effect of riboflavin on collagen fibril formation revealed by the following methods: differential scanning microcalorimetry, rheology, and STEM images. The collagen used was extracted from calf skin that contains type I collagen similar to that found in the eye. Spin trapping experiments on collagen/hyaluronic acid/riboflavin solutions evidenced the formation of ROS species by electron paramagnetic resonance measurements.

【 授权许可】

Unknown