Neuromuscular interaction is required for neurotrophins-mediated locomotor recovery following treadmill training in rat spinal cord injury

PeerJ

Chuanming Dong¹  Hongxing Wang²  Xiangzhe Li²  Qinfeng Wu²  Tong Wang²  Yana Cao³  Qinghua Wang⁴  Weifeng Tong⁵  Chunlei Shan⁶ 

[1] Department of Anatomy and Neurobiology, Nantong University, Nantong, Jiangsu, China;Department of Rehabilitation Medicine, the First Affiliated Hospital of Nanjing Medical University,Nanjing, Jiangsu, China;Jiangsu Province Hospital of TCM, Nanjing, Jiangsu, China;Laboratory Animal Center, Nantong University, Nantong, Jiangsu, China;Research Center for Neurobiological, Xuzhou Medical Collage, Xuzhou, Jiangsu, China;School of Rehabilitation Science, Shanghai University of Traditional Chinese Medicine, Shanghai, China;

关键词: Treadmill training;    Spinal cord injury;    Neurotrophins;    Motor neurons;    Neuromuscular activity;    BDNF;   

DOI  :  10.7717/peerj.2025

来源: DOAJ

【 摘 要 】

Recent results have shown that exercise training promotes the recovery of injured rat distal spinal cords, but are still unclear about the function of skeletal muscle in this process. Herein, rats with incomplete thoracic (T10) spinal cord injuries (SCI) with a dual spinal lesion model were subjected to four weeks of treadmill training and then were treated with complete spinal transection at T8. We found that treadmill training allowed the retention of hind limb motor function after incomplete SCI, even with a heavy load after complete spinal transection. Moreover, treadmill training alleviated the secondary injury in distal lumbar spinal motor neurons, and enhanced BDNF/TrkB expression in the lumbar spinal cord. To discover the influence of skeletal muscle contractile activity on motor function and gene expression, we adopted botulinum toxin A (BTX-A) to block the neuromuscular activity of the rat gastrocnemius muscle. BTX-A treatment inhibited the effects of treadmill training on motor function and BDNF/TrKB expression. These results indicated that treadmill training through the skeletal muscle-motor nerve-spinal cord retrograde pathway regulated neuralplasticity in the mammalian central nervous system, which induced the expression of related neurotrophins and promoted motor function recovery.

【 授权许可】

Unknown