The Identification of Small RNAs Differentially Expressed in Apple Buds Reveals a Potential Role of the Mir159-MYB Regulatory Module during Dormancy

Plants

Marcos Mota do Carmo Costa¹  Roberto Coiti Togawa¹  Priscila Grynberg¹  Bruno Huettel²  Isabelle Farrera³  Fernando Andrés³  Evelyne Costes³  Julio Garighan³  Etienne Dvorak³  Joan Estevan³  Karine Loridon³  Gautier Sarah³  Julie Leclercq³ 

[1] Bioinformatica Laboratory, Embrapa Recursos Genéticos e Biotecnologia—Cenargen, Brasilia 02372, Brazil;Genome Centre, Max Planck Institute for Plant Breeding Research, D-50829 Cologne, Germany;UMR AGAP Institute, Institut Agro, CIRAD, INRAE, University of Montpellier, F-34398 Montpellier, France;

关键词: dormancy;    apple tree;    small RNAs;    miR159;   

DOI  :  10.3390/plants10122665

来源: DOAJ

【 摘 要 】

Winter dormancy is an adaptative mechanism that temperate and boreal trees have developed to protect their meristems against low temperatures. In apple trees (Malus domestica), cold temperatures induce bud dormancy at the end of summer/beginning of the fall. Apple buds stay dormant during winter until they are exposed to a period of cold, after which they can resume growth (budbreak) and initiate flowering in response to warmer temperatures in spring. It is well-known that small RNAs modulate temperature responses in many plant species, but however, how small RNAs are involved in genetic networks of temperature-mediated dormancy control in fruit tree species remains unclear. Here, we have made use of a recently developed ARGONAUTE (AGO)-purification technique to isolate small RNAs from apple buds. A small RNA-seq experiment resulted in the identification of 17 micro RNAs (miRNAs) that change their pattern of expression in apple buds during dormancy. Furthermore, the functional analysis of their predicted target genes suggests a main role of the 17 miRNAs in phenylpropanoid biosynthesis, gene regulation, plant development and growth, and response to stimulus. Finally, we studied the conservation of the Arabidopsis thaliana regulatory miR159-MYB module in apple in the context of the plant hormone abscisic acid homeostasis.

【 授权许可】

Unknown