The mosquito microbiome includes habitat-specific but rare symbionts

Computational and Structural Biotechnology Journal

A. Murat Eren¹  Mathieu Sicard²  Nonito Pages³  Ignace Rakotoarivony³  Sarah Chakloute³  Florentin Constancias⁴  Mylène Weill⁵  Florian Trigodet⁵  Albane Marie⁶  Gregory L'Ambert⁶  Patrick Makoundou⁷  Julie Reveillaud⁷  Hans Schrieke⁸  Loïs Maignien⁹ 

[1] CIRAD, UMR ASTRE, Guadeloupe, France;Josephine Bay Paul Center for Comparative Molecular Biology and Evolution, Marine Biological Laboratory, Woods Hole, MA, USA;ASTRE, University of Montpellier, CIRAD, INRAE, Montpellier, France;CIRAD, UMR Qualisud, Montpellier, France;Department of Medicine, University of Chicago, Chicago, IL, USA;EID Méditerranée, 165 Avenue Paul Rimbaud, 34184 Montpellier, France;ISEM, University of Montpellier, CNRS, EPHE, IRD, Montpellier, France;MIVEGEC, University of Montpellier, INRAE, CNRS, IRD, Montpellier, France;Univ Brest, CNRS, IFREMER, Microbiology of Extreme Environments Laboratory, Plouzané, France;

关键词: Microbiome;    Mosquitoes;    Symbionts;    Microdiversity;    Oligotyping;   

DOI  :  

来源: DOAJ

【 摘 要 】

Microbial communities are known to influence mosquito lifestyles by modifying essential metabolic and behavioral processes that affect reproduction, development, immunity, digestion, egg survival, and the ability to transmit pathogens. Many studies have used 16S rRNA gene amplicons to characterize mosquito microbiota and investigate factors that influence host-microbiota dynamics. However, a relatively low taxonomic resolution due to clustering methods based on arbitrary threshold and the overall dominance of Wolbachia or Asaia symbionts obscured the investigation of rare members of mosquito microbiota in previous studies. Here, we used high resolution Shannon entropy-based oligotyping approach to analyze the microbiota of Culex pipiens, Culex quinquefasciatus and Aedes individuals from continental Southern France and overseas Guadeloupe as well as from laboratories with or without antibiotics treatment. Our experimental design that resulted in a series of mosquito samples with a gradient of Wolbachia density and relative abundance along with high-resolution analyses of amplicon sequences enabled the recovery of a robust signal from typically less accessible bacterial taxa. Our data confirm species-specific mosquito-bacteria associations with geography as a primary factor that influences bacterial community structure. But interestingly, they also reveal co-occurring symbiotic bacterial variants within single individuals for both Elizabethkingia and Erwinia genera, distinct and specific Asaia and Chryseobacterium in continental and overseas territories, and a putative rare Wolbachia variant. Overall, our study reveals the presence of previously overlooked microdiversity and multiple closely related symbiotic strains within mosquito individuals with a remarkable habitat-specificity.

【 授权许可】

Unknown