Akkermansia muciniphila-Derived Extracellular Vesicles as a Mucosal Delivery Vector for Amelioration of Obesity in Mice

Frontiers in Microbiology

Shohre Khatami¹  Hamid Reza Moradi²  Farzam Vaziri⁴  Seyed Davar Siadat⁴  Fatemeh Ashrafian⁴  Shima Hadifar⁴  Rezvan Yaghoubfar⁴  Shahrbanoo Keshavarz Azizi Raftar⁴  Sara Ahmadi Badi⁴  Ava Behrouzi⁴  Arefeh Shahriary⁴  Arezou Lari⁵ 

[1]Department of Biochemistry, Pasteur Institute of Iran, Tehran, Iran

[2]Department of Histology and Embryology Group, Basic Sciences, Faculty of Veterinary Medicine, Shiraz University, Shiraz, Iran

[3]Department of Mycobacteriology and Pulmonary Research, Pasteur Institute of Iran, Tehran, Iran

[4]Microbiology Research Center, Pasteur Institute of Iran, Tehran, Iran

[5]Systems Biomedicine Unit, Pasteur Institute of Iran, Tehran, Iran

关键词: gut microbiota;    Akkermansia muciniphila;    extracellular vesicles;    toll-like receptors;    tight junction;    peroxisome proliferator-activated receptors;   

DOI  :  10.3389/fmicb.2019.02155

来源: DOAJ

【 摘 要 】

Recent evidence suggests that probiotics can restore the mucosal barrier integrity, ameliorate inflammation, and promote homeostasis required for metabolism in obesity by affecting the gut microbiota composition. In this study, we investigated the effect of Akkermansia muciniphila and its extracellular vesicles (EVs) on obesity-related genes in microarray datasets and evaluated the cell line and C57BL/6 mice by conducting RT-PCR and ELISA assays. A. muciniphila-derived EVs caused a more significant loss in body and fat weight of high-fat diet (HFD)-fed mice, compared with the bacterium itself. Moreover, treatment with A. muciniphila and EVs had significant effects on lipid metabolism and expression of inflammatory markers in adipose tissues. Both treatments improved the intestinal barrier integrity, inflammation, energy balance, and blood parameters (i.e., lipid profile and glucose level). Our findings showed that A. muciniphila-derived EVs contain various biomolecules, which can have a positive impact on obesity by affecting the involved genes. Also, our results showed that A. muciniphila and its EVs had a significant relationship with intestinal homeostasis, which highlights their positive role in obesity treatment. In conclusion, A. muciniphila-derived EVs can be used as new therapeutic strategies to ameliorate HFD-induced obesity by affecting various mechanisms.

【 授权许可】

Unknown