Genetically Encoded Tools for Research of Cell Signaling and Metabolism under Brain Hypoxia

Antioxidants

AlexanderI. Kostyuk¹  ElenaS. Fetisova¹  DmitryS. Bilan¹  OlegV. Podgorny¹  IlyaV. Kelmanson¹  AleksandraD. Kokova¹  VsevolodV. Belousov¹ 

[1] Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, 117997 Moscow, Russia;

关键词: oxygen;    hypoxia;    HIF;    genetically encoded biosensors;    fluorescent proteins;    luciferase;   

DOI  :  10.3390/antiox9060516

来源: DOAJ

【 摘 要 】

Hypoxia is characterized by low oxygen content in the tissues. The central nervous system (CNS) is highly vulnerable to a lack of oxygen. Prolonged hypoxia leads to the death of brain cells, which underlies the development of many pathological conditions. Despite the relevance of the topic, different approaches used to study the molecular mechanisms of hypoxia have many limitations. One promising lead is the use of various genetically encoded tools that allow for the observation of intracellular parameters in living systems. In the first part of this review, we provide the classification of oxygen/hypoxia reporters as well as describe other genetically encoded reporters for various metabolic and redox parameters that could be implemented in hypoxia studies. In the second part, we discuss the advantages and disadvantages of the primary hypoxia model systems and highlight inspiring examples of research in which these experimental settings were combined with genetically encoded reporters.

【 授权许可】

Unknown