Adenosine isolated from Grifola gargal promotes glucose uptake via PI3K and AMPK signalling pathways in skeletal muscle cells

Journal of Functional Foods

Danyao Ge¹  Hitoshi Ashida²  Kunio Imai³  Hirotaka Katsuzaki⁴  Takashi Mishima⁴  Esteban C. Gabazza⁵  Yasukiyo Yoshioka⁶  Etsuko Harada⁷ 

[1] Department of Immunology, Mie University Graduate School of Medicine, Tsu, Mie 514-8507, Japan;Department of Immunology, Mie University Graduate School of Medicine, Tsu, Mie 514-8507, Japan;Graduate School of Agricultural Science, Kobe University, Kobe, Hyogo 651-8501, Japan;Graduate School of Bioresources, Mie University, Tsu, Mie 514-8507, Japan;Graduate School of Regional Innovation Studies, Mie University, Tsu, Mie 514-8507, Japan;Graduate School of Science, Technology and Innovation, Kobe University, Kobe, Hyogo 657-8501, Japan;Iwade Research Institute of Mycology Co. Ltd., Tsu, Mie 514-0012, Japan;

关键词: Adenosine;    AMPK;    Glucose uptake;    GLUT4;    Grifola gargal;    Muscle;   

DOI  :  

来源: DOAJ

【 摘 要 】

Grifola gargal, an edible mushroom originating in southern Argentina and Chile, is known to have such beneficial health effects as antioxidant and anti-inflammatory activities. A low molecular weight fraction from Grifola gargal (GLF) decreased fasting blood glucose levels in high-fat diet-induced diabetic mice. This implicated Grifola gargal as a functional food to prevent type 2 diabetes mellitus (T2DM). To address the unknown underlying molecular mechanisms, we investigated, using L6 myotubes, whether GLF promoted GLUT4 translocation to the plasma membrane and its related signalling pathways. In addition, adenosine was isolated and identified the active compound in GLF to increase glucose uptake and promote GLUT4 translocation in L6 myotubes. Adenosine activated PI3K/Akt signalling and phosphorylation of AMPK, by binding to the adenosine receptor A1. Thus, Grifola gargal is a promising functional food to prevent post-prandial hyperglycaemia and T2DM by promoting glucose uptake in muscle.

【 授权许可】

Unknown