Weighted Gene Co-expression Network Analysis Identifies Specific Modules and Hub Genes Related to Subacute Ruminal Acidosis

Frontiers in Veterinary Science

Xueqing Yue¹  Bingnan Gao¹  Yizhe Cui¹  Juan J. Loor²  Xu Wei³  Chuang Xu⁴  Qiuju Wang⁵  Xiaoxia Dai⁶ 

[1] College of Animal Science and Technology, Heilongjiang Bayi Agricultural University, Daqing, China;Department of Animal Sciences, Division of Nutritional Sciences, University of Illinois, Urbana, IL, United States;Department of Biosystems, Division of Animal and Human Health Engineering, KU Leuven, Leuven, Belgium;Heilongjiang Provincial Key Laboratory of Prevention and Control of Bovine Diseases, Daqing, China;Key Laboratory of Low-Carbon Green Agriculture in Northeastern China, Ministry of Agriculture and Rural Affairs P. R. China, Heilongjiang Bayi Agricultural University, Daqing, China;The Royal Veterinary College, University of London, London, United Kingdom;

关键词: subacute ruminal acidosis;    bioinformatic analysis;    differentially expressed genes;    inflammatory response;    transmembrane signaling;   

DOI  :  10.3389/fvets.2022.897714

来源: DOAJ

【 摘 要 】

Weighted gene co-expression network analysis (WGCNA) was used to understand the pathogenesis of subacute ruminal acidosis (SARA) and identify potential genes related to the disease. Microarray data from dataset GSE143765, which included 22 cows with and nine cows without SARA, were downloaded from the NCBI Gene Expression Omnibus (GEO). Results of WGCNA identified highly correlated modules of sample genes, and Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analyses allowed further biological insights into SARA-related modules. The protein-protein interaction (PPI) network, modules from the PPI network, and cistron annotation enrichment of modules were also analyzed. A total of 14,590 DEGs were used for the WGCNA. Construction of a protein-protein network identified DCXR, MMP15, and MMP17 as hub genes. Functional annotation showed that DCXR mainly exhibited L-xylulose reductase (NADP+) activity, glucose metabolic process, xylulose metabolic process, and carbonyl reductase (NADPH) activity, which are involved in the pentose and glucuronate interconversion pathways. MMP15 and MMP17 mainly have a collagen catabolic process. Overall, the results of this study aid the clarification of the biological and metabolic processes associated with SARA at the molecular level. The data highlight potential mechanisms for the future development of intervention strategies to reduce or alleviate the risk of SARA.

【 授权许可】

Unknown