Characterisation of Collagen Re-Modelling in Localised Prostate Cancer Using Second-Generation Harmonic Imaging and Transrectal Ultrasound Shear Wave Elastography

Cancers

Jennifer Wilson¹  Chidozie N. Ogbonnaya²  Ghulam Nabi²  Wael Ageeli²  Xinyu Zhang³  Yuting Ling⁴  Chunhui Li⁵ 

[1] Department of Pathology, Ninewells Hospital, Dundee DD1 9SY, UK;Division of Imaging Sciences and Technology, School of Medicine, University of Dundee, Ninewells Hospital, Dundee DD1 9SY, UK;Division of Population Health and Genomics, School of Medicine, University of Dundee, Dundee DD1 9SY, UK;Imaging Science and Technology, School of Medicine, University of Dundee, Dundee DD1 9SY, UK;School of Science and Engineering, University of Dundee, Dundee DD1 4HN, UK;

关键词: prostate;    collagen;    second-generation harmonics;    shear wave;    elastography;    ultrasound;   

DOI  :  10.3390/cancers13215553

来源: DOAJ

【 摘 要 】

Prostate cancer has a poor prognosis and high mortality rate due to metastases. Extracellular matrix (ECM) re-modelling and stroma composition have been linked to cancer progression, including key components of cell migration, tumour metastasis, and tissue modulus. Moreover, collagens are one of the most significant components of the extracellular matrix and have been ascribed to many aspects of neoplastic transformation. This study characterises collagen re-modelling around localised prostate cancer using the second harmonic generation of collagen (SHG), genotyping and ultrasound shear wave elastography (USWE) measured modulus in men with clinically localised prostate cancer. Tempo-sequence assay for gene expression of COL1A1 and COL3A1 was used to confirm the expression of collagen. Second-harmonic generation imaging and genotyping of ECM around prostate cancer showed changes in content, orientation, and type of collagen according to Gleason grades (cancer aggressivity), and this correlated with the tissue modulus measured by USWE in kilopascals. Furthermore, there were clear differences between collagen orientation and type around normal and cancer tissues.

【 授权许可】

Unknown