Ganoderma zonatum Is the Causal Agent of Basal Stem Rot in Oil Palm in Colombia

Journal of Fungi

María Camila Rodríguez¹  Hernán Mauricio Romero¹  Carmenza Montoya¹  Anuar Morales²  Sandra Yulieth Castillo²  León Franky Zúñiga²  Héctor Camilo Medina²  Luis Felipe González²  Yuri Adriana Mestizo²  Greicy Andrea Sarria² 

[1] Biology and Plant Breeding Program, Colombian Oil Palm Research Center—Cenipalma, Bogotá 111211, Colombia;Pest and Disease Program, Colombian Oil Palm Research Center—Cenipalma, Bogotá 111211, Colombia;

关键词: Elaeis guineensis;    pathogenicity;    phylogenetic analysis;   

DOI  :  10.3390/jof8030230

来源: DOAJ

【 摘 要 】

Basal stem rot (BSR), caused by Ganoderma spp., is one of the most important emerging oil palm diseases in Colombia, and is restricted to two oil palm production areas in the country. To identify the causal agent of the disease, basidiocarp of oil palms affected by BSR were used to prepare isolates, and their pathogenicity was then assessed in pre-nursery plants. Four-month-old oil palm seedlings were inoculated with rubber wood (Hevea brasiliensis) blocks colonized with dikaryotic mycelia of Ganoderma. The incidence, severity, and symptoms of the pathogen were assessed. A multiregional analysis (ITS, rpb2, and tef1-α) was carried out to identify the isolates; all isolates were determined to be Ganoderma zonatum. Phylogenetic analyses with the three regions yielded concordant phylogenetic information and supported the distinction of the isolates with high bootstrap support. Seven isolates (CPBsZN-01-29, CPBsZN-02-30, CPBsZN-03-31, CPBsZN-04-34, CPBsZN-05-35, CPBsZN-06-36, and CPBsZN-07-38) were pathogenic in oil palm, with incidences greater than 90% and a maximum severity of 34%, and the highest severity index was found in isolates CPBsZN-03-31, CPBsZN-04-34, and CPBsZN-06-36. The pathogen was recovered from inoculated oil palms in all cases. This study reveals the pathogenic association of Ganoderma zonatum with BSR in Colombia.

【 授权许可】

Unknown