Characterization of Strip1 Expression in Mouse Cochlear Hair Cells

Frontiers in Genetics

Renjie Chai¹  Pengjun Wang³  Yin Chen⁵  Jie Chen⁵  Xiaoli Zhang⁵  Xia Gao⁵  Ying Dong⁶  Shasha Zhang⁶  Pei Jiang⁶  Ruiying Qiang⁶  Yuan Zhang⁶  Jingru Ai⁶  Leilei Wu⁶  Xiangyu Ma⁶ 

[1] Beijing Key Laboratory of Neural Regeneration and Repair, Capital Medical University, Beijing, China;Co-Innovation Center of Neuroregeneration, Nantong University, Nantong, China;Department of Otorhinolaryngology, Affiliated Sixth People’s Hospital of Shanghai Jiao Tong University, Shanghai, China;Institute for Stem Cell and Regeneration, Chinese Academy of Sciences, Beijing, China;Jiangsu Provincial Key Medical Discipline (Laboratory), Department of Otolaryngology Head and Neck Surgery, Affiliated Drum Tower Hospital of Nanjing University Medical School, Nanjing, China;State Key Laboratory of Bioelectronics, School of Life Sciences and Technology, Jiangsu Province High-Tech Key Laboratory for Bio-Medical Research, Southeast University, Nanjing, China;

关键词: hair cell;    cochlea;    inner ear;    expression;    Strip1;   

DOI  :  10.3389/fgene.2021.625867

来源: DOAJ

【 摘 要 】

Striatin-interacting protein 1 (Strip1) is a core component of the striatin interacting phosphatase and kinase (STRIPAK) complex, which is involved in embryogenesis and development, circadian rhythms, type 2 diabetes, and cancer progression. However, the expression and role of Strip1 in the mammalian cochlea remains unclear. Here we studied the expression and function of Strip1 in the mouse cochlea by using Strip1 knockout mice. We first found that the mRNA and protein expression of Strip1 increases as mice age starting from postnatal day (P) 3 and reaches its highest expression level at P30 and that the expression of Strip1 can be detected by immunofluorescent staining starting from P14 only in cochlear HCs, and not in supporting cells (SCs). Next, we crossed Strip1 heterozygous knockout (Strip +/−) mice to obtain Strip1 homozygous knockout (Strip1−/−) mice for studying the role of Strip1 in cochlear HCs. However, no Strip1−/− mice were obtained and the ratio of Strip +/− to Strip1+/+ mice per litter was about 2:1, which suggested that homozygous Strip1 knockout is embryonic lethal. We measured hearing function and counted the HC number in P30 and P60 Strip +/− mice and found that they had normal hearing ability and HC numbers compared to Strip1+/+ mice. Our study suggested that Strip1 probably play important roles in HC development and maturation, which needs further study in the future.

【 授权许可】

Unknown