Development of a Novel, Anti-idiotypic Monoclonal Anti-prolactin Antibody That Mimics the Physiological Functions of Prolactin

Asian-Australasian Journal of Animal Sciences

Ming-Long Li¹  Dian-Cai Zhang²  Shen-Tian Wang³ 

[1] China Shandong Provincial Hospital, Jinan250000, China;Dermatological Department, Yidu Central Hospital of Weifang, Weifang 262500, China;;

关键词: Prolactin;    Anti-idotypic Antibody;    Signal Transduction;   

DOI  :  10.5713/ajas.15.0541

来源: DOAJ

【 摘 要 】

In this work, we prepared a panel of monoclonal anti-idiotypic antibodies to ovine prolactin (oPRL) by the hybridoma technique. Among these antibodies, one anti-idotypic antibody (designated B7) was chosen for further characterization by a series of experiments. We first demonstrated that B7 behaved as a typical Ab2β based on a series of enzyme-linked immunosorbent assays. Subsequently, the results of a competitive receptor-binding assay confirmed that B7 could specifically bind to the prolactin receptor (PRLR) expressed on target cells. Finally, we examined its biological activities in CHO-PRLR and Nb2 cells and observed that B7 could activate Janus kinase 2-signal transducer and activator of transcription signalling in CHO-PRLR and Nb2 cells and induce BaF3 proliferation. The present study suggests that i) B7 can serve as a PRLR agonist or PRL mimic and has potential applications in regulating mammary gland development, milk production and maintenance of lactation in domestic animals and ii) B7 may be a biological reagent that can be used to explore the mechanism of PRLR-mediated intracellular signalling.

【 授权许可】

Unknown