Diffusion-Based Separation of Extracellular Vesicles by Nanoporous Membrane Chip

Biosensors

Gijung Kim¹  Hojun Kim¹  Wonjune Kim¹  Honggu Chun¹  Seonae Jang²  Daeyoung Han³  Sunghoon Kim³  Min Chul Park³ 

[1] Department of Biomedical Engineering, Korea University, Seoul 02841, Korea;Department of Biomicrosystem Technology, Korea University, Seoul 02841, Korea;Medicinal Bioconvergence Research Center, Suwon 16229, Korea;

关键词: exosomes;    diffusion-based separation;    PC membrane;    nanopore;   

DOI  :  10.3390/bios11090347

来源: DOAJ

【 摘 要 】

Extracellular vesicles (EVs) have emerged as novel biomarkers and therapeutic material. However, the small size (~200 nm) of EVs makes efficient separation challenging. Here, a physical/chemical stress-free separation of EVs based on diffusion through a nanoporous membrane chip is presented. A polycarbonate membrane with 200 nm pores, positioned between two chambers, functions as the size-selective filter. Using the chip, EVs from cell culture media and human serum were separated. The separated EVs were analyzed by nanoparticle tracking analysis (NTA), scanning electron microscopy, and immunoblotting. The experimental results proved the selective separation of EVs in cell culture media and human serum. Moreover, the diffusion-based separation showed a high yield of EVs in human serum compared to ultracentrifuge-based separation. The EV recovery rate analyzed from NTA data was 42% for cell culture media samples. We expect the developed method to be a potential tool for EV separation for diagnosis and therapy because it does not require complicated processes such as immune, chemical reaction, and external force and is scalable by increasing the nanoporous membrane size.

【 授权许可】

Unknown