Susceptibility and Multidrug Resistance Patterns of Escherichia coli Isolated from Cloacal Swabs of Live Broiler Chickens in Bangladesh

Pathogens

Ruhul Amin¹  Mst. Ismat Ara Begum²  Hossam M. Ashour³  Ahmed S. Khairalla⁴  A.T.M. Badruzzaman⁵  Md. Masudur Rahman⁵  Asmaul Husna⁵  Kannika Na Lampang⁶  Muha. Ajijur Rahman Al Azad⁶  Reinhard Fries⁷  Hafez Mohamed Hafez⁸  Mohamed E. El Zowalaty⁹ 

[1] Bangladesh Council for Scientific and Industrial Research, Rajshahi 6206, Bangladesh;Department of Animal Husbandry and Veterinary Science, Rajshahi University, Rajshahi 6205, Bangladesh;Department of Biological Sciences, College of Arts and Sciences, University of South Florida St. Petersburg, St. Petersburg, FL 33701, USA;Department of Microbiology & Immunology, Faculty of Pharmacy, Beni-Suef University, Beni-Suef 62511, Egypt;Department of Pathology, Faculty of Veterinary, Animal and Biomedical Sciences, Sylhet Agricultural University, Sylhet 3100, Bangladesh;Department of Veterinary Biosciences and Veterinary Public Health, Faculty of Veterinary Medicine, Chiang Mai University, Chiang Mai 50100, Thailand;Institute of Food Safety and Food Hygiene, Freie Universitaet Berlin, 14195 Berlin, Germany;Institute of Poultry Diseases, Faculty of Veterinary Medicine, Freie Universitaet Berlin, 14195 Berlin, Germany;Virology & Microbiology Research Group, College of Pharmacy, City University College of Ajman, Al Tallah 2, Ajman, P.O. Box 18484, United Arab Emirates;

关键词: E. coli;    antibiotics;    multi-drug resistance;    broiler chicken;    zoonotic;    Bangladesh;   

DOI  :  10.3390/pathogens8030118

来源: DOAJ

【 摘 要 】

Antimicrobial resistance is a major health problem, particularly in developing countries like Bangladesh, where there is a paucity of information on resistance patterns and prevalence of antimicrobial determinants. Therefore, the aims of this study were to investigate the prevalence of resistance, including multi-drug resistance (MDR), and the associated genetic determinants in Escherichia coli isolates from cloacal swabs of live broiler chickens in Bangladesh. Altogether, 400 cloacal swabs (200 from Rajshahi and 200 from Dhaka divisions) were randomly collected from individual chickens in 50 broiler farms. E. coli was isolated and identified using conventional bacteriological culture and biochemical methods. The isolates were further confirmed using genus-specific 16S rRNAtargeted polymerase chain reaction (PCR) primers. Antimicrobial susceptibilities and MDR of the isolates against nine different antimicrobial agents (ampicillin, erythromycin, tetracycline, gentamicin, ciprofloxacin, levofloxacin, trimethoprim-sulfamethoxazole, colistin sulphate, and streptomycin) were determined using the Kirby-Bauer disc diffusion method. Resistance determinants of E. coli to ampicillin (bla_TEM), streptomycin (aadA1), erythromycin [ere(A)], trimethoprim (dfrA1), and tetracycline [tet(A), tet(B)] were screened using PCR. Our results showed that all swab samples were positive for E. coli. The isolates were uniformly resistant to ampicillin, tetracycline, streptomycin, ciprofloxacin, erythromycin, and trimethoprim-sulphamethoxazole. The isolates exhibited highest susceptibility to colistin sulphate (73.5%), followed by gentamicin (49%), and levofloxacin (17%). All isolates were resistant to three classes of antibiotics, 204 isolates (51%) were resistant to four classes, and 56 isolates (14%) were resistant to five. The highest prevalence of antimicrobial resistance gene was recorded for tetracycline (tet(A):95.25%; tet(B):95.25%) followed by ampicillin (bla_TEM:91.25%), streptomycin (aadA1:88.25%), erythromycin (ere(A):84.75%), and trimethoprim (dfrA1:65.5%). In conclusion, surveillance for MDR bacteria in poultry is a critical piece of knowledge, which would be useful for optimizing empiric antimicrobial treatments and exploring alternative antimicrobial agents.

【 授权许可】

Unknown