Genetic Diversity of Enteric Viruses in Children under Five Years Old in Gabon

Viruses

Benjamin Mordmüller¹  Sandra Niendorf²  Daniel Eibach³  Mirabeau Mbong Ngwese⁴  C.-Thomas Bock⁴  AyolaAkim Adegnika⁴  PaulAlvyn Nguema-Moure⁴  PeterG. Kremsner⁴  ThirumalaisamyP. Velavan⁴  Steffen Borrmann⁴  GédéonPrince Manouana⁴ 

[1] Department of Medical Microbiology, Radboudumc, 6524 GA Nijmegen, The Netherlands;Division of Viral Gastroenteritis and Hepatitis Pathogens and Enteroviruses, Department of Infectious Diseases, Robert Koch Institute, 13353 Berlin, Germany;Infectious Disease Epidemiology, Bernhard Nocht Institute for Tropical Medicine, 20359 Hamburg, Germany;Institute of Tropical Medicine, Universitätsklinikum Tübingen, 72074 Tübingen, Germany;

关键词: enteric viruses;    children;    phylogenetic analysis;    diarrhea;    Gabon;   

DOI  :  10.3390/v13040545

来源: DOAJ

【 摘 要 】

Enteric viruses are the leading cause of diarrhea in children globally. Identifying viral agents and understanding their genetic diversity could help to develop effective preventive measures. This study aimed to determine the detection rate and genetic diversity of four enteric viruses in Gabonese children aged below five years. Stool samples from children <5 years with (n = 177) and without (n = 67) diarrhea were collected from April 2018 to November 2019. Norovirus, astrovirus, sapovirus, and aichivirus A were identified using PCR techniques followed by sequencing and phylogenetic analyses. At least one viral agent was identified in 23.2% and 14.9% of the symptomatic and asymptomatic participants, respectively. Norovirus (14.7%) and astrovirus (7.3%) were the most prevalent in children with diarrhea, whereas in the healthy group norovirus (9%) followed by the first reported aichivirus A in Gabon (6%) were predominant. The predominant norovirus genogroup was GII, consisting mostly of genotype GII.P31-GII.4 Sydney. Phylogenetic analysis of the 3CD region of the aichivirus A genome revealed the presence of two genotypes (A and C) in the study cohort. Astrovirus and sapovirus showed a high diversity, with five different astrovirus genotypes and four sapovirus genotypes, respectively. Our findings give new insights into the circulation and genetic diversity of enteric viruses in Gabonese children.

【 授权许可】

Unknown