The role and possible mechanism of the long noncoding RNA LINC01260 in nonalcoholic fatty liver disease

Nutrition & Metabolism

Xiaoxiao Ge¹  Tao Sun¹  Bingyang Zhang¹  Sumei Lu¹  Yanmei Zhang¹  Yongqing Li¹  Peijun Wang¹  Wanshan Ma¹  Dantong Zhang²  Peng Gao³ 

[1]Department of Laboratory Medicine, Shandong Provincial Qianfoshan Hospital, Shandong University

[2]Department of Laboratory Medicine, The First Affiliated Hospital of Shandong First Medical University

[3]Medical Research Center, The First Affiliated Hospital of Shandong First Medical University

关键词: Nonalcoholic fatty liver disease;    Long-chain noncoding RNAs;    Eukaryotic circular sequencing;    Oleic acid;    Hepatocyte steatosis;   

DOI  :  10.1186/s12986-021-00634-4

来源: DOAJ

【 摘 要 】

Abstract Objective To investigate the differential expression profile of lncRNAs in the nonalcoholic fatty liver disease (NAFLD) model induced by oleic acid (OA) and to further explore the role of LINC01260 (ENST00000255183) in NAFLD, providing theoretical support for the clinical value of lncRNAs in NAFLD. Methods OA (50 μg/mL) was used to induce steatosis in normal human LO2 hepatocytes for 48 h and was verified by Oil red O staining. Differential expression profiles of lncRNAs were obtained by eukaryotic circular sequencing (RNA/lncRNA/circRNA-seq) techniques. A gain-of-function (GOF) strategy for LINC01260 was adopted, Oil red O staining and semiquantitative analysis were combined to explore whether the GOF of LINC01260 affects LO2 cell steatosis. CeRNA-based bioinformatics analysis of lncRNAs was performed, and the enriched mRNAs were further verified. RXRB siRNAs were applied and verify its role in LINC01260 regulated OA-induced hepatocytes steatosis. Results Lipid droplets of different sizes were observed in the cells of the OA group. Absorbance in the OA group was significantly increased after isopropanol decolorization (P < 0.05). Compared with those in the control group, there were 648 lncRNAs with differential expression greater than 1 time in the OA group, of which 351 were upregulated and 297 were downregulated. Fluorescence quantitative PCR showed that the expression of LINC01260 in the OA group was downregulated by 0.35 ± 0.07-fold (P < 0.05). The formation of lipid droplets in LO2 cells of the LINC01260 GOF group decreased significantly (P < 0.05). CeRNA analysis indicated that the mRNA levels of RXRB, RNPEPL1, CD82, MADD and KLC2 were changed to different degrees. Overexpression of LINC01260 significantly induced RXRB transcription (P < 0.05) and translation, and RXRB silence attenuated the lipids decrease induced by LINC01260 overexpression. Conclusion The OA-induced NAFLD cell model has a wide range of lncRNA differential expression profiles. LINC01260 participates in the regulation of the lipid droplet formation process of NAFLD, and its overexpression can significantly inhibit the steatosis process of LO2 cells. Mechanistically, LINC01260 may act as a ceRNA to regulate the expression of RXRB, thereby affecting the adipocytokine signaling pathway.

【 授权许可】

Unknown