期刊论文详细信息
Microorganisms
Genotyping Canadian Cyclospora cayetanensis Isolates to Supplement Cyclosporiasis Outbreak Investigations
Muhammad Morshed1  Brent R. Dixon2  James D. Wasmuth3  John R. Barta4  Christine A. Yanta5  Rebecca A. Guy5  Antoine Corbeil6  Robert Needle7  Karine Thivierge8  Hervé Menan8 
[1] BC Centre for Disease Control Public Health Laboratory, Vancouver, BC V5Z 4R4, Canada;Bureau of Microbial Hazards, Food Directorate, Health Canada, Ottawa, ON K1A 0K9, Canada;Department of Ecosystem and Public Health, Faculty of Veterinary Medicine, University of Calgary, Calgary, AB T2N 4Z6, Canada;Department of Pathobiology, Ontario Veterinary College, University of Guelph, Guelph, ON N1G 2W1, Canada;National Microbiology Laboratory at Guelph, Division of Enteric Diseases, Public Health Agency of Canada, Guelph, ON N1G 3W4, Canada;Public Health Ontario, Toronto, ON M5G 1M1, Canada;Public Health and Microbiology Laboratory, Eastern Health, St John’s, NL A1A 3Z9, Canada;Québec Public Health Laboratory, Ste-Anne-de-Bellevue, QC H9X 3R5, Canada;
关键词: Cyclospora cayetanensis;    cyclosporiasis;    genotyping;    targeted amplicon deep sequencing (TADS);    Canadian outbreaks;    foodborne pathogen;   
DOI  :  10.3390/microorganisms10020447
来源: DOAJ
【 摘 要 】

Cyclospora cayetanensis is an emerging foodborne parasite that causes cyclosporiasis, an enteric disease of humans. Domestically acquired outbreaks have been reported in Canada every spring or summer since 2013. To date, investigations into the potential sources of infection have relied solely on epidemiological data. To supplement the epidemiological data with genetic information, we genotyped 169 Canadian cyclosporiasis cases from stool specimens collected from 2010 to 2021 using an existing eight-marker targeted amplicon deep (TADS) scheme specific to C. cayetanensis as previously described by the US Centers for Disease Control and Prevention (CDC). This is the first study to genotype Canadian Cyclospora cayetanensis isolates, and it focuses on evaluating the genotyping performance and genetic clustering. Genotyping information was successfully collected with at least part of one of the markers in the TADS assay for 97.9% of specimens, and 81.1% of cyclosporiasis cases met the minimum requirements to genetically cluster into 20 groups. The performance of the scheme suggests that examining cyclosporiasis cases genetically will be a valuable tool for supplementing epidemiological outbreak investigations and to minimize further infections. Further research is required to expand the number of discriminatory markers to improve genetic clustering.

【 授权许可】

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