Neuroprotective Effects of Curcumin in Methamphetamine-Induced Toxicity

Molecules

Andrea Fornasiero¹  Linda Balestrini¹  Elena Pompili²  Stefano Leone³  Larisa Ryskalin⁴  Michela Ferrucci⁴  Francesco Fornai⁴  Gloria Lazzeri⁴  Stefano Puglisi-Allegra⁵  CarlaL. Busceti⁵  Francesca Biagioni⁵ 

[1] Aliveda Laboratory S.r.l., Viale Karol Wojtyla, 19, Località Pian di Laura, 56042 Pisa, Italy;Department of Anatomy, Histology, Forensic Medicine and Orthopedics, Sapienza University of Rome, Via A. Borelli 50, 00161 Rome, Italy;Department of Biology, Roma Tre University, Viale Guglielmo Marconi 446, 00146 Rome, Italy;Department of Translational Research and New Technologies in Medicine and Surgery, University of Pisa, Via Roma 55, 56126 Pisa, Italy;Istituto di Ricovero e Cura a Carattere Scientifico (I.R.C.C.S.) Neuromed, Via Atinense 18, 86077 Pozzilli, Italy;

关键词: Curcuma longa;    natural polyphenol;    drug of abuse;    autophagy;    α-synuclein;    LC3;   

DOI  :  10.3390/molecules26092493

来源: DOAJ

【 摘 要 】

Curcumin (CUR), a natural polyphenol extracted from rhizome of the Curcuma longa L, has received great attention for its multiple potential health benefits as well as disease prevention. For instance, CUR protects against toxic agents acting on the human body, including the nervous system. In detail, CUR possesses, among others, strong effects as an autophagy activator. The present study indicates that CUR counteracts methamphetamine (METH) toxicity. Such a drug of abuse is toxic by disturbing the autophagy machinery. We profited from an unbiased, low variable cell context by using rat pheochromocytoma PC12 cell line. In such a system, a strong protection was exerted by CUR against METH toxicity. This was associated with increased autophagy flux, merging of autophagosomes with lysosomes and replenishment of autophagy vacuoles with LC3, which instead is moved out from the vacuoles by METH. This is expected to enable the autophagy machinery. In fact, while in METH-treated cells the autophagy substrates α-synuclein accumulates in the cytosol, CUR speeds up α-synuclein clearance. Under the effects of CUR LC3 penetrate in autophagy vacuoles to commit them to cell clearance and promotes the autophagy flux. The present data provide evidence that CUR counteracts the neurotoxic effects induced by METH by promoting autophagy.

【 授权许可】

Unknown