Antifungal Activity of Cyclic Tetrapeptide from Bacillus velezensis CE 100 against Plant Pathogen Colletotrichum gloeosporioides

Pathogens

Jeong-Yong Cho¹  Jae-Hyun Moon²  YoungSang Ahn²  Sang-Jae Won²  Vantha Choub²  ChawEi Htwe Maung³  KilYong Kim³  YeonSoo Han⁴ 

[1] Department of Food Science and Technology, College of Agriculture and Life Sciences, Chonnam National University, Gwangju 61186, Korea;Department of Forest Resources, College of Agriculture and Life Sciences, Chonnam National University, Gwangju 61186, Korea;Division of Agricultural and Biological Chemistry, Institute of Environmentally Friendly Agriculture, College of Agriculture and Life Sciences, Chonnam National University, Gwangju 61186, Korea;Division of Plant Biotechnology, Institute of Environmentally Friendly Agriculture, College of Agriculture and Life Sciences, Chonnam National University, Gwangju 61186, Korea;

关键词: antagonistic bacteria;    antifungal cyclic tetrapeptide;    anthracnose disease;    mycelial growth;    spore germination;    biocontrol agent;   

DOI  :  10.3390/pathogens10020209

来源: DOAJ

【 摘 要 】

The aim of this study was to investigate the antifungal activity of a cyclic tetrapeptide from Bacillus velezensis CE 100 against anthracnose-causing fungal pathogen Colletotrichum gloeosporioides. Antifungal compound produced by B. velezensis CE 100 was isolated and purified from ethyl acetate extract of B. velezensis CE 100 culture broth using octadecylsilane column chromatography. The purified compound was identified as cyclo-(prolyl-valyl-alanyl-isoleucyl) based on mass spectrometer and nuclear magnetic resonance analyses. This is the first report of the isolation of a cyclic tetrapeptide from B. velezensis CE 100 culture filtrate. Cyclic tetrapeptide displayed strong antifungal activity at concentration of 1000 µg/mL against C. gloeosporioides mycelial growth and spore germination. Our results demonstrate that the antifungal cyclic tetrapeptide from B. velezensis CE 100 has potential in bioprotection against anthracnose disease of plants caused by C. gloeosporioides.

【 授权许可】

Unknown