Serotype Distribution and Demographics of Dengue Patients in a Tertiary Hospital of Lahore, Pakistan during the 2011 Epidemic

Walailak Journal of Science and Technology

Nakhshab CHOUDHRY¹  Samina NAEEM¹  Asad Aslam KHAN²  Ashraf SULTAN²  Muhammad MUNIR²  Riffat MEHBOOB²  Fridoon Jawad AHMAD²  Muhammad SALMAN²  Irshad Hussain QURESHI²  Akmal LAEEQ²  Shehryar AHMED²  Tahira Murtaza CHEEMA² 

[1] Department of Pathology, King Edward Medical University, Lahore;Molecular Cell Biology and Genetics Center, Department of Pathology, King Edward Medical University, Lahore;

关键词: Dengue;    serotypes;    Pakistan;    DEN 2;    Lahore;    demography;   

DOI  :  10.2004/wjst.v10i3.528

来源: DOAJ

【 摘 要 】

The dengue outbreak in Lahore, Pakistan during 2011 was unprecedented in terms of its severity and magnitude. This research aims to determine the serotype distribution of the dengue virus during this outbreak and classify the patients demographically. 5ml of venous blood was drawn aseptically from 166 patients with dengue like signs to test for the virus between the months of August to November 2011. The samples were sent to CDC, Atlanta, Georgia for the purpose of molecular assays to determine their serotype. RT-PCR protocol was performed targeting the 4 dengue serotypes. Out of 166 cases, dengue infection was detected with RT-PCR in 95 cases, all infected with the same serotype DEN-2. 75 % of positive cases were males while 25 % were females. Most positive patients were in the age range of 16 - 30 years. 33 % positive cases had accompanying bleeding. This is first study during the 2011 dengue epidemic in Lahore that reports DEN-2 as the only prevalent serotype. It also indicates that more infected patients were males, adults, within the age range of 16 - 30 years, the epidemic peaked in the month of November, Dengue hemorrhagic fever (DHF) is manifested more in females, and that Ravi town was heavily hit by the dengue virus infection.

【 授权许可】

Unknown