Gynostemma pentaphyllum polysaccharide prevents the growth of h22 ascites tumour by enhancing immunity rather than cytotoxicity in mice

Food and Agricultural Immunology

Xu Chen¹  Fangli Ma²  Minghua Hu²  Xiangliang Deng³  Zhuo Wang³  Xinyue He³  Yi Xiao³  Lian Zhou⁴  Manqing Zheng⁴  Xinguo Su⁵  Zhiyan Ruan⁵ 

[1] Affiliated Hospital 1, Guangzhou University of Chinese Medicine;Infinitus Chinese Herbal Immunity Research Centre;School of Chinese Medicine, Guangdong Pharmaceutical University;School of Pharmaceutical Sciences, Guangzhou University of Chinese Medicine;School of Pharmacy, Guangdong Food & Drug Vocational College;

关键词: gynostemma pentaphyllum polysaccharide;    enhancing immunity;    h22;    t cells;    cytotoxicity;   

DOI  :  10.1080/09540105.2020.1730770

来源: DOAJ

【 摘 要 】

Polysaccharide from Gynostemma pentaphyllum Makino (GPP) could prevent H22 tumour growth and have immunostimulatory in mice, but the mechanism is largely unknown. In this study, we further investigated whether GPP prevents tumour growth by inducing cytotoxicity to the tumour cells or enhancing immunity in H22 ascites tumour-bearing mice. The results showed that GPP prevented the H22 ascites tumour growth in vivo, but showed little effect on cell viability and cycles to H22 cells in vitro. GPP improved the proportion and mitochondrial level of T cells, and promoted the secretion of IL-2 and IFN-γ in the ascites of the mice. Furthermore, GPP in vitro enhanced the cell viability and promoted activation (in particular T cells), proliferation, and the secretion of IL-2 and IFN-γ of lymphocyte. Taken together, our results demonstrated that GPP prevents H22 ascites tumour growth by enhancing immunity rather than cytotoxicity in mice.

【 授权许可】

Unknown