Anti-Autophagy Mechanism of Zhi Gan Prescription Based on Network Pharmacology in Nonalcoholic Steatohepatitis Rats

Frontiers in Pharmacology

Yunxi Ji¹  Beilei Li²  Chufeng Qin³  Lichuan Luo⁴  Yijie Lou⁵  Jingwen Lou⁵  Yusheng Cui⁵  Zhuofan Weng⁵  Zhaojun Wang⁵  Cuiting Weng⁵  Chenxin Liu⁵  Li Jiang⁶ 

[1] Department of General Practice, The First Affiliated Hospital of Zhejiang Chinese Medical University, Hangzhou, China;Department of Traditional Chinese Medicine, Changan Hospital, Xian, China;School of Basic Medical Sciences, Zhejiang Chinese Medical University, Hangzhou, China;School of Humanities and Management, Zhejiang Chinese Medical University, Hangzhou, China;The First School of Clinical Medicine, Zhejiang Chinese Medical University, Hangzhou, China;Yuanben Health Management Co. LTD, Hangzhou, China;

关键词: network pharmacology;    non-alcoholic steatohepatitis;    zhi gan prescription;    autophagy;    molecular mechanisms;   

DOI  :  10.3389/fphar.2021.708479

来源: DOAJ

【 摘 要 】

Background and Aims: Zhi Gan prescription (ZGP) has been clinically proven to exert a favorable therapeutic effect on nonalcoholic steatohepatitis (NASH). This study purpose to reveal the underlying molecular mechanisms of ZGP action in NASH.Methods: Systematic network pharmacology was used to identify bioactive components, potential targets, and the underlying mechanism of ZGP action in NASH. High fat (HF)-induced NASH model rats were used to assess the effect of ZGP against NASH, and to verify the possible molecular mechanisms as predicted by network pharmacology.Results: A total of 138 active components and 366 potential targets were acquired in ZGP. In addition, 823 targets of NASH were also screened. In vivo experiments showed that ZGP significantly improved the symptoms in HF-induced NASH rats. qRT-PCR and western blot analyses showed that ZGP could regulate the hub genes, PTEN, IL-6 and TNF in NASH model rats. In addition, ZGP suppressed mitochondrial autophagy through mitochondrial fusion and fission via the PINK/Parkin pathway.Conclusion: ZGP exerts its effects on NASH through mitochondrial autophagy. These findings provide novel insights into the mechanisms of ZGP in NASH.

【 授权许可】

Unknown