Molecular Detection of Tick-Borne Pathogens in American Bison (Bison bison) at El Uno Ecological Reserve, Janos, Chihuahua, Mexico

Pathogens

Julio V. Figueroa-Millán¹  José J. Lira-Amaya¹  Martha P. Olivas-Sánchez²  Javier A. Garza-Hernández²  Diana M. Beristain-Ruiz³  Gabriel Pons-Monarrez³  Carlos A. Rodríguez-Alarcón³  Cuauhcihuatl Vital-García³  Samuel Flores-Ceballos⁴  Juan R. Sánchez-Ayala⁴ 

[1] CENID-Salud Animal e Inocuidad, Instituto Nacional de Investigaciones Forestales, Agrícolas y Pecuarias, Cuernavaca-Cuautla 8534, Progreso, Jiutepec CP 62574, Mexico;Departamento de Ciencias Químico-Biológicas, Universidad Autónoma de Ciudad Juárez, Anillo Envolvente y Estocolmo s/n Colonia Progresista AP 1729-D Cd. Juárez, Chihuahua CP 32310, Mexico;Departamento de Ciencias Veterinarias, Universidad Autónoma de Ciudad Juárez, Anillo Envolvente y Estocolmo s/n Colonia Progresista AP 1729-D Cd. Juárez, Chihuahua CP 32310, Mexico;Facultad de Medicina Veterinaria y Zootecnia, Universidad Nacional Autónoma de México, Ciudad Universitaria, México D. F. CP 04510, Mexico;

关键词: Bison bison;    ticks;    North American prairies;    tick-borne diseases;   

DOI  :  10.3390/pathogens10111428

来源: DOAJ

【 摘 要 】

American bison (Bison bison) is listed as near-threatened and in danger of extinction in Mexico. Recent studies have demonstrated the presence of several emerging pathogens at the Janos Biosphere Reserve (JBR), inhabited by one wild herd of American bison. Blood samples were collected from 26 American bison in the JBR. We tested for the presence of Anaplasma marginale, Babesia bigemina, B. bovis, Borrelia burgdorferi sensu lato, and Rickettsia rickettsii DNA using nested and semi-nested PCR protocols performing duplicates in two different laboratories. Results showed three animals (11.5%) positive for B. burgdorferi s. l., three more (11.5%) for Rickettsia rickettsii, and four (19.2%) for B. bovis. Two individuals were co-infected with B. burgdorferi s. l. and B. bovis. We found no animals positive for A. marginale and B. bigemina. This is the first report in America of R. rickettsii in American bison. American bison has been described as an important reservoir for pathogens of zoonotic and veterinary importance; thus, the presence of tick-borne pathogen DNA in the JBR American bison indicates the importance of continuous wildlife health surveys.

【 授权许可】

Unknown