Application of α-Syn Real-Time Quaking-Induced Conversion for Brain and Skin Specimens of the Chinese Patients With Parkinson’s Disease

Frontiers in Aging Neuroscience

Cao Chen¹  Qi Shi²  Yue Huang³  Ling Jiao⁴  Xiao-Ping Dong⁵  Kang Xiao⁶  Dong-Dong Chen⁶  Li-Ping Gao⁶ 

[1] Center for Biosafety Mega-Science, Chinese Academy of Sciences, Wuhan, China;China Academy of Chinese Medical Sciences, Beijing, China;Department of Neurology, China National Clinical Research Center for Neurological Diseases, Beijing Tiantan Hospital, Capital Medical University, Beijing, China;Department of Neurology, The Affiliated Hospital of Guizhou Medical University, Guiyang, China;Shanghai Institute of Infectious Disease and Biosafety, Shanghai, China;State Key Laboratory for Infectious Disease Prevention and Control, Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Zhejiang University, Beijing, China;

关键词: RT-QuIC;    Parkinson’s disease (PD);    prion disease (PrD);    α-synuclein;    PrPsc;   

DOI  :  10.3389/fnagi.2022.898516

来源: DOAJ

【 摘 要 】

The real-time quaking-induced conversion (RT-QuIC) assay has been developed and used as an in vitro diagnostic tool for Parkinson’s disease (PD). In this study, we established α-Syn RT-QuIC using recombinant human α-Syn as the substrate. All 5 brain homogenates of neuropathological PD cases and 13 skin homogenates of clinical PD cases showed positive results, whereas all the samples of negative controls remain negative. Meantime, randomly selected 6 skin samples of PD cases and 6 skin samples of sCJD cases showed negative in opposite prion RT-QuIC and α-Syn RT-QuIC. Our α-Syn RT-QuIC showed dose-dependent manner between the lag times and peak ThT fluorescent values. Additionally, the detecting limitation was about 10–7 dilution for brain tissues and 10–6 for skins. Those data indicate a reliable specificity and good sensitivity of the established α-Syn RT-QuIC in identifying and amplifying the misfolded α-Syn in brain and skin tissues of patients with PD.

【 授权许可】

Unknown