High prevalence of carbapenemase, AmpC β-lactamase and aminoglycoside resistance genes in extended-spectrum β-lactamase-positive uropathogens from Northern India

Journal of Global Antimicrobial Resistance

Birson Ingti¹  Deepjyoti Paul¹  Amitabha Bhattacharjee¹  Arunabha Sarkar²  Jyotsna Kapil³  Ashish Kumar Singh⁴  Nilu Pradhan⁴  Hare Krishna Tiwari⁴  Varsha Rani Gajamer⁴ 

[1] Department of Microbiology, Assam University, Assam, India;Department of Microbiology, North Bengal Medical College, Siliguri, West Bengal, India;Department of Microbiology, Sikkim Manipal Institute of Medical Sciences, Gangtok, Sikkim, India;Department of Microbiology, Sikkim University, Gangtok, Sikkim, India;

关键词: UTI;    Multidrug-resistant;    Antimicrobial resistance genes;    NDM;    ESBL;    Northern India;   

DOI  :  

来源: DOAJ

【 摘 要 】

Objectives: This study investigated the occurrence of extended-spectrum β-lactamase (ESBL) genes coexisting with carbapenemase, AmpC and aminoglycoside resistance gene in uropathogens in India. Methods: Antimicrobial susceptibility testing was performed by disk diffusion. Antimicrobial resistance genes were detected by multiplex PCR. Results: Of 1516 consecutive urine samples, 454 (29.9%) showed significant bacteriuria with a single micro-organism, predominantly Escherichia coli (n = 343), followed by Klebsiella pneumoniae (n = 92), Pseudomonas aeruginosa (n = 10) and Proteus mirabilis (n = 9). Among the uropathogens, 61 ESBL-producers were identified containing blaCTX-M-15 (n = 32), blaCTX-M-15 + blaOXA-2 (n = 15), blaCTX-M-15 + blaOXA-2 + blaTEM-1 (n = 6), blaOXA-2 (n = 5), blaOXA-2 + blaSHV-76 (n = 1), blaTEM-1+blaSHV-76 (n = 1) and blaTEM-1 (n = 1). All ESBL genes were located on horizontally transferable plasmids of incompatibility types HI1, I1, FIA + FIB, FIA and Y. Among the 61 ESBL-producers, 59 harboured carbapenemase genes, including blaNDM-5 (n = 48), blaNDM-5 + blaOXA-48 (n = 5), blaNDM-5 + blaIMP (n = 5) and blaNDM-5+blaIMP + blaVIM (n = 1). ESBL-producing uropathogens also harboured 16S rRNA methylase genes, including rmtB (n = 9), rmtA (n = 4), rmtC (n = 1) and armA (n = 1). ESBL-positive isolates also contained AmpC genes, including blaCIT (n = 8) and blaDHA-1 (n = 1). Imipenem and gentamicin had the lowest resistance rates against the uropathogens. Conclusion: This is the first report showing the high prevalence of carbapenemases in ESBL-positive isolates in this area. Regular surveillance for such resistance mechanisms will be useful for health personnel to treat infections by these multidrug-resistant pathogens.

【 授权许可】

Unknown