PCL-Coated Multi-Substituted Calcium Phosphate Bone Scaffolds with Enhanced Properties

Materials

Gloria Gallego-Ferrer¹  Maja Antunović²  Hrvoje Ivanković²  Marica Ivanković²  Leonard Bauer² 

[1] Centre for Biomaterials and Tissue Engineering (CBIT), Universitat Politècnica de València, 46022 Valencia, Spain;Faculty of Chemical Engineering and Technology, University of Zagreb, HR-10001 Zagreb, Croatia;

关键词: scaffold;    hydroxyapatite;    whitlockite;    Mg;    Sr;    co-substitution;   

DOI  :  10.3390/ma14164403

来源: DOAJ

【 摘 要 】

Ionic substitutions within the hydroxyapatite lattice are a widely used approach to mimic the chemical composition of the bone mineral. In this work, Sr-substituted and Mg- and Sr-co-substituted calcium phosphate (CaP) scaffolds, with various levels of strontium and magnesium substitution, were prepared using the hydrothermal method at 200 °C. Calcium carbonate skeletons of cuttlefish bone, ammonium dihydrogenphosphate (NH₄H₂PO₄), strontium nitrate (Sr(NO₃)₂), and magnesium perchlorate (Mg(ClO₄)₂) were used as reagents. Materials were characterized by means of X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FTIR) and scanning electron microscopy (SEM). Whole powder pattern decomposition refinements of XRD data indicated that increased magnesium content in the Mg- and Sr-co-substituted scaffolds was related to an increased proportion of the whitlockite (WH) phase in the biphasic hydroxyapatite (HAp)/WH scaffolds. In addition, refinements indicate that Sr²⁺ ions have replaced Ca²⁺ sites in the WH phase. Furthermore, PCL-coated Mg-substituted and Sr- and Mg-co-substituted scaffolds, with the HAp:WH wt. ratio of 90:10 were prepared by vacuum impregnation. Results of compression tests showed a positive impact of the WH phase and PCL coating on the mechanical properties of scaffolds. Human mesenchymal stem cells (hMSCs) were cultured on composite scaffolds in an osteogenic medium for 21 days. Immunohistochemical staining showed that Mg-Sr-CaP/PCL scaffold exhibited higher expression of collagen type I than the Mg-CaP/PCL scaffold, indicating the positive effect of Sr²⁺ ions on the differentiation of hMSCs, in concordance with histology results. Reverse transcription-quantitative polymerase chain reaction (RT-qPCR) analysis confirmed an early stage of osteogenic differentiation.

【 授权许可】

Unknown