Green synthesis of iron nanoparticles from Ulva lactuca and bactericidal activity against enteropathogens

Journal of King Saud University: Science

G. Johnsi Christobel¹  Perumal Anantharaman²  Asha D.V. Bensy³  Karnan Muthusamy³  Ahmed Alfarhan⁴ 

[1] Corresponding author.;Department of Botany and Microbiology, College of Science, King Saud University, P.O. Box 2455, Riyadh 11451, Saudi Arabia;Department of Botany, Nesamony Memorial Christian College, Kanyakumari District, Affiliated to Manonmaniam Sundaranar University, Abishekapatti, Tirunelveli 627 012, Tamilnadu, India;Grassland and Forages Division, National Institute of Animal Science, Rural Development Administration, Cheonan 31000, Republic of Korea;

关键词: Marine algae;    Aqueous extract;    Capping agent;    Iron nanoparticles;    Antibacterial;    Anticancer;   

DOI  :  

来源: DOAJ

【 摘 要 】

Antimicrobial resistance has emerged as one of the major public-health issues in recent times. The multi-drug resistant opportunistic bacterial pathogens, Staphylococcus aureus, Escherichia coli, and Salmonella typhimurium cause urinary tract, respiratory infections and diarrhea. The prevalence in the hospital environment increases multi-drug resistant bacteria and beta-lactam antibiotics (carbapenems) are highly effective against these pathogens, however development of drug resistance against these antibiotics may lead to increased mortality. Hence, it is of utmost significance to develop alternative antibiotics against these drug-resistant bacteria. In this study, we have synthesized iron nanoparticles using the water extract of marine algae (Ulva lactuca) collected from Tamilnadu coast, India. The nanoparticles were characterized by UV-spectroscopy, transform infrared spectroscopy, and Scanning electron microscopy. The green synthesized nanoparticles have the size of 30–40 nm and have potent biological activities. The synthesized nanoparticles showed anticancer activity against HeLa and DLD-1 cell lines. The methanol extract of U. lactuca showed maximum activity against E. coli (24 ± 2 mm), followed by S. typhimurium (23 ± 1 mm), B. cereus (19 ± 1 mm), P. vulgaris (17 ± 2 mm), and S. aureus (16 ± 2 mm). Antibacterial disc diffusion analysis of nanoparticles showed improved activites than algal extract. The iron nanoparticles showed activity against S. aureus (24 ± 1 mm), E. coli (29 ± 1 mm), and S. typhimurium (31 ± 2 mm). The minimum inhibitory concentration values of nanoparticles against diarrhoea causing bacteria varied widely. U. lactuca extract was highly effective against S. typhimurium (25 µg/mL), followed by E. coli (30 µg/mL), and S. aureus (45 µg/mL). These results clearly demonstrate that iron nanoparticles could be developed as alternate therapeutics against carbapenems resistant bacteria and against types of cancers.

【 授权许可】

Unknown