Interaction between potyvirus and crinivirus in sweet potato

Pesquisa Agropecuária Tropical

Julia Martino¹  Liliana Di Feo²  Antonella Vilanova Perez²  Andrea Peña Malavera³  Sofía Solange Flamarique⁴ 

[1] Consejo Nacional de Investigaciones Científicas y Técnicas, Córdoba, Argentina.;Instituto de Patología Vegetal, Centro de Investigaciones Agropecuarias, Instituto Nacional de Tecnología Agropecuaria, Córdoba, Argentina.;Instituto de Tecnología Agroindustrial del Noroeste Argentino, Estación Experimental Agroindustrial Obispo Colombres, Consejo Nacional de Investigaciones Científicas y Técnicas, Tucumán, Argentina. ;Universidad Nacional de Córdoba, Facultad de Ciencias Exactas, Físicas y Naturales, Córdoba, Argentina. ;

关键词: ipomoea batatas;    spfmv;    spvg;    spcsv;    virus concentration;   

DOI  :  10.1590/1983-40632020v5058735

来源: DOAJ

【 摘 要 】

Sweet potato, in Argentina, is affected by the “encrespamiento amarillo”, a viral disease in which seven viruses are involved, among them two potyvirus (sweet potato feathery mottle virus - SPFMV and sweet potato virus G - SPVG) and a crinivirus (sweet potato chlorotic stunt virus - SPCSV). This research aimed to study the interactionbetweenSPFMVandSPVGwithSPCSVinsweetpotato.Twosweetpotatocultivars(GemandArapeyINIA)andIpomoeasetosaasanindicatorsusceptibleplantweretestedassingle (SPFMV or SPVG), double (SPFMV + SPVG, SPFMV + SPCSV or SPVG + SPCSV) or triple (SPFMV + SPVG + SPCSV) grafts. Both potyviruses were purified and the viral concentrations in the plant tissues were quantified by the DAS-Elisa method. The viruses and their severities were evaluated at 7, 15, 21, 30 and 35 days post-inoculation. A synergistic effect was observed with the three viruses in the indicator plant. The viral concentration increase was 50 times for SPFMV (day 35) and two times for SPVG (day 21) in the Gem cultivar, and 1.89 times for SPFMV (day 35) and three times for SPVG (day 7) in the Arapey INIA. For multiple infections, theindicatorplantandtheGemcultivarexhibitedsynergisticsymptoms and increase in the viral titers, with a higher severity and variability of the symptoms. Co-infections such as SPFMV + SPVG showed characteristic potyvirus symptoms, without increasing the viralconcentrations;tripleco-infectionsexhibitedviralcomplexsymptoms, with increase in the potyvirus titers; and the symptoms were mild or imperceptible in the simple infections.

【 授权许可】

Unknown