Expression Profiles and Biological Roles of miR-196a in Swine

Genes

Meihang Li¹  Guoxi Li¹  Shuai Liu¹  Gongshe Yang²  Yang Qiu³  Xiaomin Ning³  Yanjie Li³ 

[1] F University, Yangling 712100, China;College of Animal Science and Veterinary Medicine, Henan Agricultural University, Zhengzhou 450002, China;;Laboratory of Animal Fat Deposition and Muscle Development, College of Animal Science and Technology, Northwest A &

关键词: miR-196a;    swine;    expression;    adipocyte;    proliferation;    differentiation;   

DOI  :  10.3390/genes7020005

来源: DOAJ

【 摘 要 】

MicroRNAs (miRNAs) are a class of small non-coding RNA molecules, which play important roles in animals by targeting mRNA transcripts for translational repression. Recent studies have demonstrated that miRNAs are involved in regulation of adipocyte development. The expression of miR-196a in different porcine tissues and developing fat tissues was detected, and gene ontology (GO) term enrichment was then used to predict the expression profiles and potential biological roles of miR-196a in swine. To further verify the roles of miR-196a in porcine adipocyte development, a recombinant adenovirus encoding miR-196a gene (Ad-miR-196a) was constructed and used to study the effect of miR-196a on preadipocyte proliferation and differentiation. Here, our data demonstrate that miR-196a displays a tissue-specific expression pattern and has comprehensive biological roles in swine, especially in adipose development. In addition, overexpression of miR-196a had no effect on preadipocyte proliferation, but induced preadipocyte differentiation by increasing expression of adipocyte specific markers, lipid accumulation and triglyceride content. These data represent the first demonstration of miR-196a expression profiles and roles in swine, thereby providing valuable insight into the functions of miR-196a in adipocyte biology.

【 授权许可】

Unknown