Direct estimation of reference intervals for thyroid parameters in the Republic of Srpska

Journal of Medical Biochemistry

Stojanović Darja¹  Petković Mira²  Mirjanić-Azarić Bosa³  Avram Sanja³  Stojaković-Jelisavac Tanja³  Ignjatović Svetlana⁴  Stojanov Marina⁵  Bogavac-Stanojević Nataša⁵ 

[1] Health Centre Laktasi, Republic of Srpska, Bosnia and Herzegovina;Institute for Sport and Occupational Medicine Banja Luka, Bosnia and Herzegovina;University Clinical Centre of the Republic of Srpska, Banja Luka, Bosnia and Herzegovina;University of Belgrade, Faculty of Pharmacy, Institute of Medical Biochemistry + Clinical Center of Serbia, 5Center for Medical Biochemistry, Belgrade;University of Belgrade, Faculty of Pharmacy, Institute of Medical Biochemistry;

关键词: reference values;    tsh;    thyroid hormones;   

DOI  :  

来源: DOAJ

【 摘 要 】

Background: The aim of this study was to determine the reference values for thyrotropin (TSH), thyroid hormones (total and free thyroxine, T4 and fT4; total and free triiodothyronine, T3 and fT3), thyroglobulin (Tg) and thyroid antibodies (thyroid peroxidase, TPOAb and thyroglobulin antibody, TgAb) in the population of the Republic of Srpska. Methods: A total of 250 euthyroid subjects were enrolled in this study. A direct method for choosing reference subjects was used to establish reference intervals. The hormones and thyroid antibodies were measured by an electrochemiluminescence immunoassay method (ECLIA, Roche Diagnostics, Mannheim, Germany). We calculated the reference intervals by MedCalc, version 12.1.4.0 (MedCalc software, Belgium) as recommended by the IFCC (CLSI C28-A3). Results: Using guidelines recommended by the National Academy of Clinical Biochemistry (NACB) and based on standard statistical approaches, the reference intervals derived for TSH, fT4, T4, fT3, T3 were 0.75-5.32 mIU/L, 12.29-20.03 pmol/L, 73.49-126,30 nmol/L, 4.11-6.32 pmol/L, 1.15-2.32 nmol/L and for Tg, TPOAb, TgAb were 3.63-26.00 mg/L, <18.02 mIU/L, < 98.00 mIU/L, respectively. We found a significant difference (p< 0.05) in TSH and fT3 values between different age groups as well as in T4, fT4 and fT3 values between gender groups. Conclusions: The established reference values for the population of the Republic of Srpska were significantly different from the values recommended by the manufacturer of reagents (Roche Diagnostics). Our results showed that a laboratory needs to establish its own reference values in order to set up a proper diagnosis, as well as to treat patients successfully.

【 授权许可】

Unknown